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dc.contributor.author
Stockert, Juan C.
dc.contributor.author
Herkovits, Jorge
dc.date.available
2024-08-30T11:48:23Z
dc.date.issued
2003-11
dc.identifier.citation
Stockert, Juan C.; Herkovits, Jorge; Photodynamic toxicity and its prevention by antioxidative agents in Bufo arenarum embryos; Elsevier Ireland; Toxicology; 192; 2-3; 11-2003; 211-218
dc.identifier.issn
0300-483X
dc.identifier.uri
http://hdl.handle.net/11336/243329
dc.description.abstract
In this work we describe an experimental model to evaluate the photodynamic toxicity on amphibian embryos, as well as the protective effect of antioxidants against the lethal oxidative stress induced by photosensitization. Bufo arenarum embryos were treated with 10 mg/l methylene blue (MB) in AMPHITOX solution for 72 h and then irradiated with a red laser or white light for variable times. Both light sources affected the survival of MB-treated animals and lethal effects occurred within the initial 12 h post-irradiation. For white light irradiation, the most effective phototoxic condition in our study, the LD10, 50 and 90 at 6 h post-irradiation corresponded to 13.57, 19.87 and 29.10 J/cm2, respectively. To explore the action of antioxidants against the photogenerated oxidative stress, MB-treated embryos were incubated with 1 mM glutathione (GSH) or ascorbic acid (AA) during 48 h before irradiation. For GSH and 21.6 J/cm2 irradiation, the survival increased from 20 to 90%, whereas 100% survival was achieved with AA even after 43.2 J/cm2 irradiation. These results indicate that both the lethal photodynamic effect and its prevention by antioxidants can be evaluated by means of a simple toxicity test employing amphibian embryos.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Ireland
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Amphibian embryo
dc.subject
Oxidative stress
dc.subject
Protection
dc.subject
Phototoxicity
dc.subject.classification
Otras Ciencias de la Tierra y relacionadas con el Medio Ambiente
dc.subject.classification
Ciencias de la Tierra y relacionadas con el Medio Ambiente
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Photodynamic toxicity and its prevention by antioxidative agents in Bufo arenarum embryos
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2024-08-30T11:27:38Z
dc.journal.volume
192
dc.journal.number
2-3
dc.journal.pagination
211-218
dc.journal.pais
Irlanda
dc.description.fil
Fil: Stockert, Juan C.. Fundación Pro Salud y Medio Ambiente. Instituto de Ciencias Ambientales y Salud; Argentina
dc.description.fil
Fil: Herkovits, Jorge. Fundación Pro Salud y Medio Ambiente. Instituto de Ciencias Ambientales y Salud; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.journal.title
Toxicology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0300483X03003342
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/S0300-483X(03)00334-2
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