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dc.contributor.author
Herkovits, Jorge
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dc.contributor.author
Perez Coll, Cristina Silvia
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dc.contributor.author
Stockert, Juan C.
dc.contributor.author
Blázquez, Alfonso
dc.date.available
2024-08-30T11:48:13Z
dc.date.issued
2007-12
dc.identifier.citation
Herkovits, Jorge; Perez Coll, Cristina Silvia; Stockert, Juan C.; Blázquez, Alfonso; The screening of photodynamic toxicity of dyes by means of a bioassay using amphibian embryos; Dr Jyoti Garg; Research Journal Of Chemistry And Environment; 11; 12-2007; 86-91
dc.identifier.issn
0972-0626
dc.identifier.uri
http://hdl.handle.net/11336/243328
dc.description.abstract
The photodynamic action is exerted by photogenerated reactive oxygen species (ROS) produced after cellular accumulation of a photosensitizer and irradiation with visible light. AMPHITOX test was employed successfully for standardized hazard assessment studies including phototoxic effects as well as the possibility to prevent/reduce adverse effects of different environmental agents. The main purpose of this study was to evaluate the toxicity and phototoxicity of 6 dyes and to establish the relationship between adverse effects and possible photodynamic activity. For this purpose the NOEC-48 h values were evaluated by means of the AMPHITOX test conducted with Bufo arenarum embryos at the end of their development (stage 25) for the following dyes: acridine orange (AO), phloxin (Ph), phloxin B (PhB), rose bengal B (RB), toluidine blue O (TB), and pyronine Y (PY), dissolved in AMPHITOX solution (AS). In previous studies, it was determined that irradiation with white light at a dose of 43.2 J/cm2 (72 mW/cm2 for 10 min) is appropriate to explore the photodynamic action of chemicals. Thus, for the photodynamic experiments, embryos were treated with a range of concentrations bellow the NOEC values and after 48 h of exposure they were thoroughly washed and irradiated. The NOEC-48 h values (in mg/L) for chemical toxicity and NOEC-48 h values after light irradiation were: AO 1 and 0.5; PhB 1 and 0.7; RB 5 and 1; TB 10 and 5; Ph 10 and 10; PY 20 and 20, respectively. The survival of control embryos (maintained in AS and irradiated for 10 min) was not affected. By comparing the chemical/photochemical toxicity ratios of these substances it results that the order for photodynamic action could be expressed as RB > TB > AO > PhB, the remaining dyes showing no phototoxicity. These results point out the possibility to evaluate the photodynamic effect of chemicals by means of a simple toxicity test employing amphibian embryos.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Dr Jyoti Garg
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
photodynamic action
dc.subject
dyes
dc.subject.classification
Otros Tópicos Biológicos
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
The screening of photodynamic toxicity of dyes by means of a bioassay using amphibian embryos
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2024-08-30T11:27:26Z
dc.journal.volume
11
dc.journal.pagination
86-91
dc.journal.pais
Estados Unidos
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dc.description.fil
Fil: Herkovits, Jorge. Fundación Pro Salud y Medio Ambiente; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Perez Coll, Cristina Silvia. Fundación Pro Salud y Medio Ambiente; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Martín; Argentina
dc.description.fil
Fil: Stockert, Juan C.. Fundación Pro Salud y Medio Ambiente; Argentina. Universidad Autónoma de Madrid; España
dc.description.fil
Fil: Blázquez, Alfonso. Universidad Autónoma de Madrid; España
dc.journal.title
Research Journal Of Chemistry And Environment
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