Artículo
Green synthesis and antibacterial-antibiofilm properties of biogenic silver nanoparticles
Ferreira, Maria Laura
; Lazzarini Behrmann, Irene Constanza; Daniel, María Alejandra; Sosa, Griselda Leonor
; Owusu, Ethel; Parkin, Ivan P.; Candal, Roberto Jorge
; Allan, Elaine; Vullo, Diana Lia
![Icon](/themes/CONICETDigital/images/conicet.png)
![Icon](/themes/CONICETDigital/images/conicet.png)
![Icon](/themes/CONICETDigital/images/conicet.png)
![Icon](/themes/CONICETDigital/images/conicet.png)
Fecha de publicación:
12/2024
Editorial:
Elsevier Science SA
Revista:
Environmental Nanotechnology, Monitoring & Management
ISSN:
2215-1532
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
The biosynthesis of metallic nanoparticles is gaining prominence as an alternative to traditional physicochemical methods, offering several advantages such as simplicity, non-toxicity, lower energy requirements and short reaction times leading to environmentally sustainable processes. The aims of this work were: to study the extracellular biosynthesis of silver nanoparticles (AgNPs) by Pseudomonas extremaustralis 2E-UNGS, to characterise the shape, monodispersity and size of AgNPs, to explore their antimicrobial and antibiofilm activities, and to evaluate the role of nitrate reductase activity in the biosynthesis process. The novelty of this work relies on the development of a green and sustainable method for the synthesis of stable AgNPs with optimal properties for potential applications in antimicrobial materials, especially when incorporated into polymeric matrices or used as agrochemical substitutes. Optimal conditions for the biosynthesis of spherical AgNPs were determined to be pH 7, 38 ◦C, 4 h of darkness and 120 rpm using stationary phase culture supernatants of P. extremaustralis 2EUNGS. The involvement of extracellular nitrate reductase in AgNP biosynthesis was confirmed by enzymatic assays and supported by bioinformatics analysis, which identified the presence of the napA2 gene linked to the nirBD cluster. Antimicrobial assays demonstrated the inhibitory effect of AgNPs against both Gram-positive and Gram-negative bacteria, including Pseudomonas aeruginosa PA01 in both planktonic and biofilm states. In addition, the potential application of AgNPs in innovative antibacterial polymers was explored by incorporating them into polyurethane matrices either alone (PU-AgNP) or in combination with crystal violet as a photosensitizer (PU-AgNP-CV). Subsequent inoculation with a clinical isolate of Pseudomonas aeruginosa resulted in significant reductions in viable bacterial counts on both PU-AgNP-CV and PU-AgNP. Biogenic AgNPs showed antibacterial and antibiofilm properties for new antimicrobial material development.
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos (IIIA)
Articulos de INSTITUTO DE INVESTIGACION E INGENIERIA AMBIENTAL
Articulos de INSTITUTO DE INVESTIGACION E INGENIERIA AMBIENTAL
Articulos(SEDE CENTRAL)
Articulos de SEDE CENTRAL
Articulos de SEDE CENTRAL
Citación
Ferreira, Maria Laura; Lazzarini Behrmann, Irene Constanza; Daniel, María Alejandra; Sosa, Griselda Leonor; Owusu, Ethel; et al.; Green synthesis and antibacterial-antibiofilm properties of biogenic silver nanoparticles; Elsevier Science SA; Environmental Nanotechnology, Monitoring & Management; 22; 12-2024; 1-13
Compartir
Altmétricas