Repositorio Institucional
Repositorio Institucional
CONICET Digital
  • Inicio
  • EXPLORAR
    • AUTORES
    • DISCIPLINAS
    • COMUNIDADES
  • Estadísticas
  • Novedades
    • Noticias
    • Boletines
  • Ayuda
    • General
    • Datos de investigación
  • Acerca de
    • CONICET Digital
    • Equipo
    • Red Federal
  • Contacto
JavaScript is disabled for your browser. Some features of this site may not work without it.
  • INFORMACIÓN GENERAL
  • RESUMEN
  • ESTADISTICAS
 
Artículo

Differentiation of Nolana and Sclerophylax (Solanaceae) by means of heterochromatin and rDNA patterns

Lujea, Noelia Carolina; Chiarini, Franco EzequielIcon
Fecha de publicación: 05/01/2017
Editorial: Taylor & Francis
Revista: New Zealand Journal of Botany
ISSN: 0028-825X
e-ISSN: 1175-8643
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Bioquímica y Biología Molecular

Resumen

Within Solanaceae, Nolana L. f. and Sclerophylax Miers are two genera that have deserved special attention because of their rare anatomical characteristics. However, they are poorly known from the cytogenetic perspective. In order to discuss their chromosome features in an evolutionary context, classical staining, chromomycin A3/4′ -6-diamidino-2-phenylindole (CMA/ DAPI) fluorescent banding and in situ hybridisation (FISH) with probes for the 18-5.8-26S and the 5S rDNA loci were applied to root tips of germinating seeds. All the species presented the chromosome number 2n = 24. Karyotypes were highly symmetric, with most chromosomes being metacentric and with a maximum of three submetacentric pairs in N. divaricata. The CMA/DAPI banding technique, assayed for the first time in Sclerophylax, showed CMA+ /DAPI− bands associated with nucleolar organiser regions (NORs) in the first metacentric chromosome pair in each species. The FISH technique (applied to four species of Sclerophylax and, for the first time, in one species of Nolana) showed that the 18-5.8-26S loci coincide with CMA+ /DAPI− bands. Three Sclerophylax species presented two pairs of 5S signals, whereas S. adnatifolia showed three. The rDNA loci resulted as asyntenic in Sclerophylax, but were localised in the same chromosome in N. divaricata. Despite the morphological peculiarities of Nolana and Sclerophylax, the chromosome number and karyotype features are consistent with the position of the two genera within the ‘x = 12 clade’, while the number and position of rDNA loci established the chromosome rearrangements, suggesting different evolutionary pathways with respect to their closest relatives, Lycium and Jaborosa.
Palabras clave: Heterochromatin , Karyotype , Nolana , Rdna , Sclerophyllax , South America
Ver el registro completo
 
Archivos asociados
Tamaño: 1.854Mb
Formato: PDF
.
Solicitar
Licencia
info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/24302
DOI: http://dx.doi.org/10.1080/0028825X.2016.1269812
URL: http://www.tandfonline.com/doi/full/10.1080/0028825X.2016.1269812
Colecciones
Articulos(IMBIV)
Articulos de INST.MULTIDISCIPL.DE BIOLOGIA VEGETAL (P)
Citación
Lujea, Noelia Carolina; Chiarini, Franco Ezequiel; Differentiation of Nolana and Sclerophylax (Solanaceae) by means of heterochromatin and rDNA patterns; Taylor & Francis; New Zealand Journal of Botany; 55; 2; 5-1-2017; 163-177
Compartir
Altmétricas
 
Estadísticas
Visualizaciones: 119
Descargas: 0

Enviar por e-mail
Separar cada destinatario (hasta 5) con punto y coma.
  • Facebook
  • Twitter
  • Instagram
  • YouTube
  • Sound Cloud

Los contenidos del CONICET están licenciados bajo Creative Commons Reconocimiento 2.5 Argentina License

Ministerio
https://www.conicet.gov.ar/ - CONICET

Inicio

Explorar

  • Autores
  • Disciplinas
  • Comunidades

Estadísticas

Novedades

  • Noticias
  • Boletines

Ayuda

Acerca de

  • CONICET Digital
  • Equipo
  • Red Federal

Contacto

Godoy Cruz 2290 (C1425FQB) CABA – República Argentina – Tel: +5411 4899-5400 repositorio@conicet.gov.ar
TÉRMINOS Y CONDICIONES