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dc.contributor.author
Braglia, Chiara
dc.contributor.author
Alberoni, Daniele
dc.contributor.author
Garrido, Paula Melisa
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dc.contributor.author
Porrini, Martín Pablo
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dc.contributor.author
Baffoni, Loredana
dc.contributor.author
Scott, Dara
dc.contributor.author
Eguaras, Martin Javier
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dc.contributor.author
Di Gioia, Diana
dc.contributor.author
Mifsud, David
dc.date.available
2024-08-22T10:17:38Z
dc.date.issued
2024-05-13
dc.identifier.citation
Braglia, Chiara; Alberoni, Daniele; Garrido, Paula Melisa; Porrini, Martín Pablo; Baffoni, Loredana; et al.; Vairimorpha (Nosema) ceranae can promote Serratia development in honeybee gut: an underrated threat for bees?; Frontiers Media; Frontiers in Cellular and Infection Microbiology; 14; 13-5-2024; 1-11
dc.identifier.issn
2235-2988
dc.identifier.uri
http://hdl.handle.net/11336/243001
dc.description.abstract
The genus Serratia harbors opportunistic pathogenic species, among which Serratia marcescens is pathogenic for honeybees although little studied. Recently, virulent strains of S. marcescens colonizing the Varroa destructor mite’s mouth were found vectored into the honeybee body, leading to septicemia and death. Serratia also occurs as an opportunistic pathogen in the honeybee’s gut with a low absolute abundance. The Serratia population seems controlled by the host immune system, but its presence may represent a hidden threat, ready to arise when honeybees are weakened by biotic and abiotic stressors. To shed light on the Serratia pathogen, this research aims at studying Serratia’s development dynamics in the honeybee body and its interactions with the co-occurring fungal pathogen Vairimorpha ceranae. Firstly, the degree of pathogenicity and the ability to permeate the gut epithelial barrier of three Serratia strains, isolated from honeybees and belonging to different species (S. marcescens, Serratia liquefaciens, and Serratia nematodiphila), were assessed by artificial inoculation of newborn honeybees with different Serratia doses (104, 106, and 108 cells/mL). The absolute abundance of Serratia in the gut and in the hemocoel was assessed in qPCR with primers targeting the luxS gene. Moreover, the absolute abundance of Serratia was assessed in the gut of honeybees infected with V. ceranae at different development stages and supplied with beneficial microorganisms and fumagillin. Our results showed that all tested Serratia strains could pass through the gut epithelial barrier and proliferate in the hemocoel, with S. marcescens being the most pathogenic. Moreover, under cage conditions, Serratia better proliferates when a V. ceranae infection is co-occurring, with a positive and significant correlation. Finally, fumagillin and some of the tested beneficial microorganisms could control both Serratia and Vairimorpha development. Our findings suggest a correlation between the two pathogens under laboratory conditions, a co-occurring infection that should be taken into consideration by researches when testing antimicrobial compounds active against V. ceranae, and the related honeybees survival rate. Moreover, our findings suggest a positive control of Serratia by the environmental microorganism Apilactobacillus kunkeei in a in vivo model, confirming the potential of this specie as beneficial bacteria for honeybees.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Frontiers Media
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
SERRATIA
dc.subject
NOSEMOSIS
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NOSEMA CERANAE
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FUMAGILLIN
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BENEFICIAL BACTERIA
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APILACTOBACILLUS
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Otras Ciencias Veterinarias
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dc.subject.classification
Ciencias Veterinarias
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dc.subject.classification
CIENCIAS AGRÍCOLAS
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dc.title
Vairimorpha (Nosema) ceranae can promote Serratia development in honeybee gut: an underrated threat for bees?
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2024-07-10T14:13:56Z
dc.journal.volume
14
dc.journal.pagination
1-11
dc.journal.pais
Suiza
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dc.description.fil
Fil: Braglia, Chiara. Universidad de Bologna; Italia
dc.description.fil
Fil: Alberoni, Daniele. Universidad de Bologna; Italia
dc.description.fil
Fil: Garrido, Paula Melisa. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina
dc.description.fil
Fil: Porrini, Martín Pablo. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina
dc.description.fil
Fil: Baffoni, Loredana. Universidad de Bologna; Italia
dc.description.fil
Fil: Scott, Dara. No especifíca;
dc.description.fil
Fil: Eguaras, Martin Javier. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina
dc.description.fil
Fil: Di Gioia, Diana. Universidad de Bologna; Italia
dc.description.fil
Fil: Mifsud, David. No especifíca;
dc.journal.title
Frontiers in Cellular and Infection Microbiology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fcimb.2024.1323157/full
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.3389/fcimb.2024.1323157
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