Mostrar el registro sencillo del ítem

dc.contributor.author
Martín, Pedro  
dc.contributor.author
Moncada, Melisa  
dc.contributor.author
Enrique, Nicolas Jorge  
dc.contributor.author
Asuaje, Agustín  
dc.contributor.author
Valdez Capuccino, Juan Manuel  
dc.contributor.author
González, Carlos  
dc.contributor.author
Milesi, Veronica  
dc.date.available
2017-09-14T19:10:42Z  
dc.date.issued
2013-12-05  
dc.identifier.citation
Martín, Pedro; Moncada, Melisa; Enrique, Nicolas Jorge; Asuaje, Agustín; Valdez Capuccino, Juan Manuel; et al.; Arachidonic acid activation of BKCa (Slo1) channels associated to the β1-subunit in human vascular smooth muscle cells; Springer; Pflugers Archiv-european Journal Of Physiology; 466; 9; 5-12-2013; 1779-1792  
dc.identifier.issn
0031-6768  
dc.identifier.uri
http://hdl.handle.net/11336/24281  
dc.description.abstract
Arachidonic acid (AA) is a polyunsaturated fatty acid involved in a complex network of cell signaling. It is well known that this fatty acid can directly modulate several cellular target structures, among them, ion channels. We explored the effects of AA on high conductance Ca2+- and voltagedependent K+ channel (BKCa) in vascular smooth muscle cells (VSMCs) where the presence of β1-subunit was functionally demonstrated by lithocholic acid activation. Using patchclamp technique, we show at the single channel level that 10 μM AA increases the open probability (Po) of BKCa channels tenfold, mainly by a reduction of closed dwell times. AA also induces a left-shift in Po versus voltage curves without modifying their steepness. Furthermore, AA accelerates the kinetics of the voltage channel activation by a fourfold reduction in latencies to first channel opening. When AA was tested on BKCa channel expressed in HEK cells with or without the β1-subunit, activation only occurs in presence of the modulatory subunit. These results contribute to highlight the molecular mechanism of AA-dependent BKCa activation. We conclude that AA itself selectively activates the β1- associated BKCa channel, destabilizing its closed state probably by interacting with the β1-subunit, without modifying the channel voltage sensitivity. Since BKCa channels physiologically contribute to regulation of VSMCs contractility and blood pressure, we used the whole-cell configuration to show that AA is able to activate these channels, inducing significant cell hyperpolarization that can lead to VSMCs relaxation.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Springer  
dc.rights
info:eu-repo/semantics/restrictedAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
Pufas  
dc.subject
Fatty Acid  
dc.subject
Omega-6 Polyunsaturated Fatty Acid  
dc.subject
Human Umbilical Artery  
dc.subject
Patch-Clamp  
dc.subject
Single Channel  
dc.subject.classification
Bioquímica y Biología Molecular  
dc.subject.classification
Medicina Básica  
dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD  
dc.title
Arachidonic acid activation of BKCa (Slo1) channels associated to the β1-subunit in human vascular smooth muscle cells  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2017-09-01T17:45:24Z  
dc.journal.volume
466  
dc.journal.number
9  
dc.journal.pagination
1779-1792  
dc.journal.pais
Alemania  
dc.journal.ciudad
Berlin  
dc.description.fil
Fil: Martín, Pedro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnol.conicet - la Plata. Centro de Endocrinología Exp.y Aplicada (i). Grupo Vinculado Cenexa-fcex-unlp; Argentina  
dc.description.fil
Fil: Moncada, Melisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnol.conicet - la Plata. Centro de Endocrinología Exp.y Aplicada (i). Grupo Vinculado Cenexa-fcex-unlp; Argentina  
dc.description.fil
Fil: Enrique, Nicolas Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnol.conicet - la Plata. Centro de Endocrinología Exp.y Aplicada (i). Grupo Vinculado Cenexa-fcex-unlp; Argentina  
dc.description.fil
Fil: Asuaje, Agustín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnol.conicet - la Plata. Centro de Endocrinología Exp.y Aplicada (i). Grupo Vinculado Cenexa-fcex-unlp; Argentina  
dc.description.fil
Fil: Valdez Capuccino, Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnol.conicet - la Plata. Centro de Endocrinología Exp.y Aplicada (i). Grupo Vinculado Cenexa-fcex-unlp; Argentina  
dc.description.fil
Fil: González, Carlos. Pontificia Universidad Catolica de Valparaiso; Chile  
dc.description.fil
Fil: Milesi, Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnol.conicet - la Plata. Centro de Endocrinología Exp.y Aplicada (i). Grupo Vinculado Cenexa-fcex-unlp; Argentina  
dc.journal.title
Pflugers Archiv-european Journal Of Physiology  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs00424-013-1422-x  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/s00424-013-1422-x