Mostrar el registro sencillo del ítem
dc.contributor.author
Merchante, Catharina
dc.contributor.author
Vallarino, Jose G.
dc.contributor.author
Osorio, Sonia
dc.contributor.author
Aragüez, Irene
dc.contributor.author
Villarreal, Natalia Marina
dc.contributor.author
Ariza, Maria T.
dc.contributor.author
Martinez, Gustavo Adolfo
dc.contributor.author
Medina Escobar, Nieves
dc.contributor.author
Civello, Pedro Marcos
dc.contributor.author
Fernie, Alisdair R.
dc.contributor.author
Botella, Miguel A.
dc.contributor.author
Valpuesta, Victoriano
dc.date.available
2017-09-14T19:09:49Z
dc.date.issued
2013-10-05
dc.identifier.citation
Merchante, Catharina; Vallarino, Jose G.; Osorio, Sonia; Aragüez, Irene; Villarreal, Natalia Marina; et al.; Ethylene is involved in strawberry fruit ripening in an organ-specific manner; Oxford University Press; Journal of Experimental Botany; 64; 14; 5-10-2013; 4421-4439
dc.identifier.issn
0022-0957
dc.identifier.uri
http://hdl.handle.net/11336/24279
dc.description.abstract
The fruit of the strawberry Fragaria×ananassa has traditionally been classified as non-climacteric because its ripening process is not governed by ethylene. However, previous studies have reported the timely endogenous production of minor amounts of ethylene by the fruit as well as the differential expression of genes of the ethylene synthesis, reception, and signalling pathways during fruit development. Mining of the Fragaria vesca genome allowed for the identification of the two main ethylene biosynthetic genes, 1-aminocyclopropane-1-carboxylic acid (ACC) synthase and ACC oxidase. Their expression pattern during fruit ripening was found to be stage and organ (achene or receptacle) specific. Strawberry plants with altered sensitivity to ethylene could be employed to unravel the role of ethylene in the ripening process of the strawberry fruit. To this end, independent lines of transgenic strawberry plants were generated that overexpress the Arabidopsis etr1-1 mutant ethylene receptor, which is a dominant negative allele, causing diminished sensitivity to ethylene. Genes involved in ethylene perception as well as in its related downstream processes, such as flavonoid biosynthesis, pectin metabolism, and volatile biosynthesis, were differently expressed in two transgenic tissues, the achene and the receptacle. The different transcriptional responsiveness of the achene and the receptacle to ethylene was also revealed by the metabolic profiling of the primary metabolites in these two organs. The free amino acid content was higher in the transgenic lines compared with the control in the mature achene, while glucose and fructose, and citric and malic acids were at lower levels. In the receptacle, the most conspicuous change in the transgenic lines was the depletion of the tricarboxylic acid cycle intermediates at the white stage of development, most probably as a consequence of diminished respiration. The results are discussed in the context of the importance of ethylene during strawberry fruit ripening.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Oxford University Press
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Ethylene
dc.subject
Fruit
dc.subject
Metabolic Profiling
dc.subject
Non-Climacteric
dc.subject
Ripening
dc.subject
Strawberry
dc.title
Ethylene is involved in strawberry fruit ripening in an organ-specific manner
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-09-14T13:54:01Z
dc.journal.volume
64
dc.journal.number
14
dc.journal.pagination
4421-4439
dc.journal.pais
Reino Unido
dc.journal.ciudad
Oxford
dc.description.fil
Fil: Merchante, Catharina. Universidad de Málaga; España
dc.description.fil
Fil: Vallarino, Jose G.. Universidad de Málaga; España
dc.description.fil
Fil: Osorio, Sonia. Universidad de Málaga; España
dc.description.fil
Fil: Aragüez, Irene. Universidad de Málaga; España
dc.description.fil
Fil: Villarreal, Natalia Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
dc.description.fil
Fil: Ariza, Maria T.. Universidad de Málaga; España
dc.description.fil
Fil: Martinez, Gustavo Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina
dc.description.fil
Fil: Medina Escobar, Nieves. Universidad de Málaga; España
dc.description.fil
Fil: Civello, Pedro Marcos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; Argentina
dc.description.fil
Fil: Fernie, Alisdair R.. Institut Max Planck Fur Molekulare Physiologie; Alemania
dc.description.fil
Fil: Botella, Miguel A.. Universidad de Málaga; España
dc.description.fil
Fil: Valpuesta, Victoriano. Universidad de Málaga; España
dc.journal.title
Journal of Experimental Botany
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1093/jxb/ert257
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/jxb/article-lookup/doi/10.1093/jxb/ert257
Archivos asociados