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dc.contributor.author
Rosli, Hernan Guillermo
dc.contributor.author
Zhen, Yi
dc.contributor.author
Pombo, Marina Alejandra
dc.contributor.author
Zhong, Silin
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Bombarely, Aureliano
dc.contributor.author
Fei, Zhangjun
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Collmer, Alan
dc.contributor.author
Martin, Gregory B.
dc.date.available
2017-09-14T19:08:21Z
dc.date.issued
2013-11
dc.identifier.citation
Rosli, Hernan Guillermo; Zhen, Yi; Pombo, Marina Alejandra; Zhong, Silin; Bombarely, Aureliano; et al.; Transcriptomics-based screen for genes induced by flagellin and repressed by pathogen effectors identifies a cell wall-associated kinase involved in plant immunity; BioMed Central; Genome Biology; 14; 11-2013; 1-15
dc.identifier.issn
1474-760X
dc.identifier.uri
http://hdl.handle.net/11336/24275
dc.description.abstract
Background: Microbe-associated molecular patterns, such as those present in bacterial flagellin, are powerful inducers of the innate immune response in plants. Successful pathogens deliver virulence proteins, termed effectors, into the plant cell where they can interfere with the immune response and promote disease. Engineering the plant immune system to enhance disease resistance requires a thorough understanding of its components. Results: We describe a high-throughput screen, using RNA sequencing and virus-induced gene silencing, to identify tomato genes whose expression is enhanced by the flagellin microbe-associated molecular pattern flgII-28, but reduced by activities of the Pseudomonas syringae pv. tomato (Pst) type III effectors AvrPto and AvrPtoB. Gene ontology terms for this category of Flagellin-induced repressed by effectors (FIRE) genes showed enrichment for genes encoding certain subfamilies of protein kinases and transcription factors. At least 25 of the FIRE genes have been implicated previously in plant immunity. Of the 92 protein kinase-encoding FIRE genes, 33 were subjected to virus-induced gene silencing and their involvement in pattern-triggered immunity was tested with a leaf-based assay. Silencing of one FIRE gene, which encodes the cell wall-associated kinase SlWAK1, compromised the plant immune response resulting in increased growth of Pst and enhanced disease symptoms. Conclusions: Our transcriptomic approach identifies FIRE genes that represent a pathogen-defined core set of immune-related genes. The analysis of this set of candidate genes led to the discovery of a cell wall-associated kinase that participates in plant defense. The FIRE genes will be useful for further elucidation of the plant immune system.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
BioMed Central
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/
dc.subject
Rna-Seq
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Pattern Triggered Immunity
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Plant Immunity
dc.subject
Type Iii Effectors
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Pseudomonas Syringae
dc.subject.classification
Bioquímica y Biología Molecular
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Transcriptomics-based screen for genes induced by flagellin and repressed by pathogen effectors identifies a cell wall-associated kinase involved in plant immunity
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-09-14T13:53:50Z
dc.journal.volume
14
dc.journal.pagination
1-15
dc.journal.pais
Reino Unido
dc.journal.ciudad
Londres
dc.description.fil
Fil: Rosli, Hernan Guillermo. Boyce Thompson Institute for Plant Research; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas ; Argentina
dc.description.fil
Fil: Zhen, Yi. Boyce Thompson Institute for Plant Research; Estados Unidos
dc.description.fil
Fil: Pombo, Marina Alejandra. Boyce Thompson Institute for Plant Research; Estados Unidos
dc.description.fil
Fil: Zhong, Silin. Boyce Thompson Institute for Plant Research; Estados Unidos. University of Hong Kong; Hong Kong
dc.description.fil
Fil: Bombarely, Aureliano. Boyce Thompson Institute for Plant Research; Estados Unidos
dc.description.fil
Fil: Fei, Zhangjun. Boyce Thompson Institute for Plant Research; Estados Unidos
dc.description.fil
Fil: Collmer, Alan. Cornell University; Estados Unidos. King Abdulaziz University; Estados Unidos
dc.description.fil
Fil: Martin, Gregory B.. Boyce Thompson Institute for Plant Research; Estados Unidos. Cornell University; Estados Unidos. King Abdulaziz University; Estados Unidos
dc.journal.title
Genome Biology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1186/gb-2013-14-12-r139
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://genomebiology.biomedcentral.com/articles/10.1186/gb-2013-14-12-r139
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