Artículo
A side-by-side comparison of different capacitation media in developing mouse sperm fertilizing ability
González, Lucas Nicolás
; Giaccagli, Maria Milagros
; Herzfeld, Jael Dafne
; Cuasnicu, Patricia Sara
; Da Ros, Vanina Gabriela
; Cohen, Debora Juana
![Icon](/themes/CONICETDigital/images/conicet.png)
![Icon](/themes/CONICETDigital/images/conicet.png)
![Icon](/themes/CONICETDigital/images/conicet.png)
![Icon](/themes/CONICETDigital/images/conicet.png)
![Icon](/themes/CONICETDigital/images/conicet.png)
![Icon](/themes/CONICETDigital/images/conicet.png)
Fecha de publicación:
06/2024
Editorial:
Springer
Revista:
Scientific Reports
ISSN:
2045-2322
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
To acquire the ability to fertilize the egg, mammalian spermatozoa must undergo a series of changes occurring within the highly synchronized and specialized environment of the female reproductive tract, collectively known as capacitation. In an attempt to replicate this process in vitro, various culture media for mouse sperm were formulated over the past decades, sharing a similar overall composition but difering mainly in ion concentrations and metabolic substrates. The widespread use of the diferent media to study the mechanisms of capacitation might hinder a comprehensive understanding of this process, as the medium could become a confounding variable in the analysis. In this context, the present side-by-side study compares the infuence of four commonly used culture media (FD, HTF and two TYH versions) on mouse sperm capacitation. We evaluated the induction of protein kinase A phosphorylation pathway, motility, hyperactivation and acrosome reaction. Additionally, in vitro fertilization and embryo development were also assessed. By analyzing these outcomes in two mouse colonies with diferent reproductive performance, our study provides critical insights to improve the global understanding of sperm function. The results obtained highlight the importance of considering variations in medium composition, and their potential implications for the future interpretation of results.
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(IBYME)
Articulos de INST.DE BIOLOGIA Y MEDICINA EXPERIMENTAL (I)
Articulos de INST.DE BIOLOGIA Y MEDICINA EXPERIMENTAL (I)
Citación
González, Lucas Nicolás; Giaccagli, Maria Milagros; Herzfeld, Jael Dafne; Cuasnicu, Patricia Sara; Da Ros, Vanina Gabriela; et al.; A side-by-side comparison of different capacitation media in developing mouse sperm fertilizing ability; Springer; Scientific Reports; 14; 1; 6-2024; 1-13
Compartir
Altmétricas