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dc.contributor.author
Benmarce, Meryem
dc.contributor.author
Haif, Assia
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Elissondo, María Celina
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dc.contributor.author
Bouaziz, Samia
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Bentahar, Assia
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Laatamna, AbdElkarim
dc.date.available
2024-08-16T13:05:42Z
dc.date.issued
2024-03-04
dc.identifier.citation
Benmarce, Meryem; Haif, Assia; Elissondo, María Celina; Bouaziz, Samia; Bentahar, Assia; et al.; Comparison of the scolicidal activity of two leaves extracts of Myrtus communis from Algeria against Echinococcus granulosus Sensu Lato Protoscoleces; Versita; Acta Parasitologica; 69; 1; 4-3-2024; 839-853
dc.identifier.issn
1230-2821
dc.identifier.uri
http://hdl.handle.net/11336/242650
dc.description.abstract
Purpose During cystic echinococcosis surgery, the use of scolicidal agents such as hypertonic saline (20%) aims to reduce the risk of infection recurrence, but most of the used agents are associated with undesirable side efects. Therefore, the use of natural scolicidal agents such as medicinal plant extracts could reduce these medical issues. The present study aimed to compare in vitro the scolicidal activity between two extracts of the medicinal plant Myrtus communis from Algeria against Echinococcus granulosus sensu lato protoscoleces. Methods The ethanolic and aqueous extraction of plant leaves was performed. Phytochemical analysis by gas chromatography–tandem mass spectrometry (GC–MS/MS), determination of total phenolic and favonoid contents, and in vitro antioxidant activity by DPPH were evaluated for both extracts. Finally, the in vitro scolicidal activity was tested by diferent concentrations. The viability was evaluated by the eosin exclusion test. Results The phytochemical analysis revealed 28 components for the ethanolic extract and 44 components for the aqueous extract. The major components were 2′-hydroxy-5′-methoxyacetophenone and 4-amino-2-methylphenol, respectively. The total phenolic and favonoid contents were 45.9±0.085 mg of gallic acid equivalent per g of extract (GAE/g E) and 16.5±0.004 mg of quercetin equivalent per g (QE/g E) for the ethanolic extract, and 36.5±0.016 mg GAE/g E and 18.2±0.023 mg QE/g E for the aqueous extract, respectively. Furthermore, ethanolic and aqueous extracts of M. communis gave a value of IC50=0.009±0.0004 mg/ml and IC50=0.012±0.0003 mg/ml for the antioxidant activity, respectively. The in vitro scolicidal activity with concentrations of 50, 75, 100, and 150 mg/ml was tested for 5, 10, 15, and 30 min, and 5, 10, 15, 30, 60, 90, and 120 min for ethanolic and aqueous extracts, respectively. The mortality rate of protoscoleces at concentrations of 100 and 150 mg/ml was 98.8 and 100%, respectively, after 5 min of exposure to the ethanolic extract, while this rate was 100% at the same concentrations only after 60 min of exposure to the aqueous extract. Conclusions The ethanolic extract showed a stronger scolicidal activity against E. granulosus s.l protoscoleces than the aqueous extract. In the future, other investigations are necessary to elucidate the mechanism of action and the possible toxicity on human cells. Moreover, experimental animal studies are required to investigate the efcacy of diferent extracts of this plant and its components as natural anti-parasitic alternatives for the treatment of human cystic echinococcosis.
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application/pdf
dc.language.iso
eng
dc.publisher
Versita
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dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Echinococcus granulosus
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Scolicidal effect
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Myrtus communis
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Ethanolic extract
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Aqueous extract
dc.subject.classification
Farmacología y Farmacia
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dc.subject.classification
Medicina Básica
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CIENCIAS MÉDICAS Y DE LA SALUD
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dc.title
Comparison of the scolicidal activity of two leaves extracts of Myrtus communis from Algeria against Echinococcus granulosus Sensu Lato Protoscoleces
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2024-07-10T14:15:23Z
dc.journal.volume
69
dc.journal.number
1
dc.journal.pagination
839-853
dc.journal.pais
Polonia
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dc.journal.ciudad
Varsovia
dc.description.fil
Fil: Benmarce, Meryem. Ferhat Abbas University; Argelia
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Fil: Haif, Assia. Ferhat Abbas University; Argelia
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Fil: Elissondo, María Celina. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina
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Fil: Bouaziz, Samia. University of M’sila; Argelia
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Fil: Bentahar, Assia. Ferhat Abbas University; Argelia
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Fil: Laatamna, AbdElkarim. University Of Djelfa; Argelia
dc.journal.title
Acta Parasitologica
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/10.1007/s11686-024-00808-z
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/s11686-024-00808-z
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