Mostrar el registro sencillo del ítem
dc.contributor.author
Pérez, María Rita
dc.contributor.author
Pellegrini, Elisabeth
dc.contributor.author
Cano Nicolau, Joel
dc.contributor.author
Gueguen, Marie Madelaine
dc.contributor.author
Le Guillou, Dounia Menouer
dc.contributor.author
Merot, Yohann
dc.contributor.author
Vaillant, Colette
dc.contributor.author
Somoza, Gustavo Manuel
dc.contributor.author
Kah, Olivier
dc.date.available
2017-09-14T18:16:50Z
dc.date.issued
2013-07-29
dc.identifier.citation
Pérez, María Rita; Pellegrini, Elisabeth; Cano Nicolau, Joel; Gueguen, Marie Madelaine; Le Guillou, Dounia Menouer; et al.; Relationships between radial glial progenitors and 5-HT neurons in the paraventricular organ of adult zebrafish – potential effects of serotonin on adult neurogenesis; Wiley Blackwell Publishing, Inc; European Journal Of Neuroscience; 38; 29-7-2013; 3292-3301
dc.identifier.issn
0953-816X
dc.identifier.uri
http://hdl.handle.net/11336/24261
dc.description.abstract
In non-mammalian vertebrates, serotonin (5-HT)-producing neurons exist in the paraventricular organ (PVO), a diencephalic structure containing cerebrospinal fluid (CSF)-contacting neurons exhibiting 5-HT or dopamine (DA) immunoreactivity. Because the brain of the adult teleost is known for its neurogenic activity supported, for a large part, by radial glial progenitors, this study addresses the origin of newborn 5-HT neurons in the hypothalamus of adult zebrafish. In this species, the PVO exhibits numerous radial glial cells (RGCs) whose somata are located at a certain distance from the ventricle. To study relationships between RGCs and 5-HT CSF-contacting neurons, we performed 5-HT immunohistochemistry in transgenic tg(cyp19a1b-GFP) zebrafish in which RGCs are labelled with GFP under the control of the cyp19a1b promoter. We show that the somata of the 5-HT neurons are located closer to the ventricle than those of RGCs. RGCs extend towards the ventricle cytoplasmic processes that form a continuous barrier along the ventricular surface. In turn, 5-HT neurons contact the CSF via processes that cross this barrier through small pores. Further experiments using proliferating cell nuclear antigen or 5-bromo-2′-deoxyuridine indicate that RGCs proliferate and give birth to 5-HT neurons migrating centripetally instead of centrifugally as in other brain regions. Furthermore, treatment of adult zebrafish with tryptophan hydroxylase inhibitor causes a significant decrease in the number of proliferating cells in the PVO, but not in the mediobasal hypothalamus. These data point to the PVO as an intriguing region in which 5-HT appears to promote genesis of 5-HT neurons that accumulate along the brain ventricles and contact the CSF.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Wiley Blackwell Publishing, Inc
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Serotonin
dc.subject
Radial Glial Cells
dc.subject
Hypothalamus
dc.subject
Dopamine
dc.subject
Fish
dc.subject.classification
Bioquímica y Biología Molecular
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Relationships between radial glial progenitors and 5-HT neurons in the paraventricular organ of adult zebrafish – potential effects of serotonin on adult neurogenesis
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-09-14T13:54:37Z
dc.journal.volume
38
dc.journal.pagination
3292-3301
dc.journal.pais
Reino Unido
dc.journal.ciudad
Londres
dc.description.fil
Fil: Pérez, María Rita. Universite de Rennes I; Francia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
dc.description.fil
Fil: Pellegrini, Elisabeth. Universite de Rennes I; Francia
dc.description.fil
Fil: Cano Nicolau, Joel. Universite de Rennes I; Francia
dc.description.fil
Fil: Gueguen, Marie Madelaine. Universite de Rennes I; Francia
dc.description.fil
Fil: Le Guillou, Dounia Menouer. Universite de Rennes I; Francia
dc.description.fil
Fil: Merot, Yohann. Universite de Rennes I; Francia
dc.description.fil
Fil: Vaillant, Colette. Universite de Rennes I; Francia
dc.description.fil
Fil: Somoza, Gustavo Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
dc.description.fil
Fil: Kah, Olivier. Universite de Rennes I; Francia
dc.journal.title
European Journal Of Neuroscience
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1111/ejn.12348
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/ejn.12348/abstract
Archivos asociados