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dc.contributor.author
Pinto, Lilian
dc.contributor.author
Schijman, Alejandro Gabriel
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dc.contributor.author
Alonso Padilla, Julio
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dc.contributor.author
Lozano, Daniel
dc.contributor.author
Cruz Torrico, Mary
dc.contributor.author
Gamba, Pietro
dc.contributor.author
Torrez, Margarita
dc.contributor.author
Lozada, Vania
dc.contributor.author
Cartagena, Karina
dc.contributor.author
Sanchez, Jareth
dc.contributor.author
Torrico, Faustino
dc.contributor.author
Gascon, Joaquim
dc.date.available
2024-08-15T15:23:55Z
dc.date.issued
2022-11-03
dc.identifier.citation
Pinto, Lilian; Schijman, Alejandro Gabriel; Alonso Padilla, Julio; Lozano, Daniel; Cruz Torrico, Mary; et al.; Molecular detection and parasite load of Trypanosoma cruzi in digestive tract tissue of Chagas disease patients affected by megacolon; Elsevier Science; Acta Tropica; 235; 3-11-2022; 1-7
dc.identifier.issn
0001-706X
dc.identifier.uri
http://hdl.handle.net/11336/242609
dc.description.abstract
Chagas disease, caused by the Trypanosoma cruzi parasite in the Americas affects ∼ 7 million people, 30% with cardiac tissue damage and 10-15% with digestive disorders. In this study, we have developed a protocol to detect the presence of the parasite and estimate its load in resected dysfunctional tissue segments of chronically infected patients with digestive megacolon. We have included samples from 43 individuals, 38/5 with positive/negative serology for Chagas disease and digestive syndromes. Samples of 1.5 to 2.0 cm2 were taken from different points of the dysfunctional digestive tract in specialized centres in Cochabamba, Bolivia. T. cruzi cultures were performed by inoculation with NNN-LIT culture medium, and genomic material was obtained from the samples for multiplex qPCR with TaqMan probes targeting satellite nuclear DNA. Cultures failed to isolate T. cruzi but qPCR reached a sensitivity of 42.1% (16/38) with all three spots and in triplicate. A new quantification methodology using synthetic satellite DNA as quantitation standard revealed parasite loads ranging from 2.2 × 102 to 1.0 × 106 satellite DNA copies/μl. Positive samples from the distal end showed a higher parasite load. The results of the present study strengthen and add further evidence to previous findings in an experimental mouse model of chronic T. cruzi infection, providing a valuable tool to improve scientific knowledge on the relevance of the digestive tract in parasite persistence, and underline the need of a better understanding of host-pathogen interaction in digestive tissues, considering pathophysiology, disease immunology and response to treatment.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science
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dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
CHAGAS DISEASE
dc.subject
TRYPANOSOMA CRUZI
dc.subject
MEGACOLON
dc.subject
MOLECULAR DIAGNOSIS
dc.subject.classification
Bioquímica y Biología Molecular
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Molecular detection and parasite load of Trypanosoma cruzi in digestive tract tissue of Chagas disease patients affected by megacolon
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2024-07-26T13:24:42Z
dc.journal.volume
235
dc.journal.pagination
1-7
dc.journal.pais
Países Bajos
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dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Pinto, Lilian. No especifíca;
dc.description.fil
Fil: Schijman, Alejandro Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
dc.description.fil
Fil: Alonso Padilla, Julio. Instituto de Salud Carlos III; España
dc.description.fil
Fil: Lozano, Daniel. Universidad Mayor de San Simón; Bolivia
dc.description.fil
Fil: Cruz Torrico, Mary. Universidad Mayor de San Simón; Bolivia
dc.description.fil
Fil: Gamba, Pietro. No especifíca;
dc.description.fil
Fil: Torrez, Margarita. No especifíca;
dc.description.fil
Fil: Lozada, Vania. Institute of Gastroenterology Bolivian Japanese; Bolivia
dc.description.fil
Fil: Cartagena, Karina. Institute of Gastroenterology Bolivian Japanese; Bolivia
dc.description.fil
Fil: Sanchez, Jareth. Hospital “Dr. Manuel Ascencio Villarroel"; Bolivia
dc.description.fil
Fil: Torrico, Faustino. Universidad Mayor de San Simón; Bolivia
dc.description.fil
Fil: Gascon, Joaquim. Instituto de Salud Carlos III; España
dc.journal.title
Acta Tropica
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0001706X22003242
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.actatropica.2022.106632
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