Artículo
A dopamine mechanism is implied in the acquisition and expression of amphetamine and stress-induced effects observed in the lymphocyte subpopulations.
Assis, Maria Amparo
; Pacchioni, Alejandra Maria
; Collino, César; Paz, Maria Constanza
; Sotomayor, Claudia Elena
; Basso, Ana María; Cancela, Liliana Marina
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Fecha de publicación:
04/2008
Editorial:
Elsevier Science
Revista:
European Journal of Pharmacology
ISSN:
0014-2999
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Drugs of abuse and stress are associated with changes in circulating cell populations and reductions in cell-mediated immune responses. Themain goal of this study was to determine the influence of repeated and acute D-amphetamine treatments on the foot-shock stress-induced effects on the peripheral lymphocyte subpopulations, and the involvement of a dopamine mechanism in the development and expression of this phenomenon. Wistar rats received an acute (5 mg/kg/day i.p.) or a repeated (2 mg/kg/day i.p. during 9 days) amphetamine treatment, and were exposed to a foot-shock stress (1 mA, 3 s) 4 days after the last amphetamine injection. Another group was administered with haloperidol (1 mg/kg/day i.p.) 15 min previous to each daily amphetamine injection or previous to the foot-shock stress session. Then, blood cells stained with monoclonal antibodies against CD3-FITC, CD8-PE and CD4-Cy-Chrome, and against CD161a-FITC, CD3-PE, and CD45RA-Cy-Crhome, were analyzed by multiparameter flow cytometry. The exposure to a foot-shock stress induced a decrease in the absolute number of peripheral lymphocytes, as well as in CD4+ and CD8+ T-cells and B-cells in acute and repeatedly amphetamine-treated rats, whereas the NK-cell population remained unchanged. Haloperidol administration previous to each drug administration or the foot-shock stress session reversed these effects. This study provides strong evidence that dopamine can play a more general role in the influence of amphetamine on the stress-induced effects on the lymphocyte subsets. the peripheral lymphocyte subpopulations, and the involvement of a dopamine mechanism in the development and expression of this phenomenon.
Palabras clave:
AMPHETAMINE
,
FOOT-SHOCK STRESS
,
DA SENSITIZATION
,
FLOW CYTOMETRY
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Articulos(CIBICI)
Articulos de CENTRO DE INV.EN BIOQUI.CLINICA E INMUNOLOGIA
Articulos de CENTRO DE INV.EN BIOQUI.CLINICA E INMUNOLOGIA
Articulos(IFEC)
Articulos de INST. DE FARMACOLOGIA EXPERIMENTAL DE CORDOBA
Articulos de INST. DE FARMACOLOGIA EXPERIMENTAL DE CORDOBA
Citación
Assis, Maria Amparo; Pacchioni, Alejandra Maria; Collino, César; Paz, Maria Constanza; Sotomayor, Claudia Elena; et al.; A dopamine mechanism is implied in the acquisition and expression of amphetamine and stress-induced effects observed in the lymphocyte subpopulations.; Elsevier Science; European Journal of Pharmacology; 584; 2-3; 4-2008; 405-414
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