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dc.contributor.author
Byrne, Alana Brooke
dc.contributor.author
Bonnin, Florencia Agustina
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López, Eduardo L.
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Polack, Fernando Pedro
dc.contributor.author
Talarico, Laura Beatriz
dc.date.available
2024-08-13T11:39:35Z
dc.date.issued
2024-06
dc.identifier.citation
Byrne, Alana Brooke; Bonnin, Florencia Agustina; López, Eduardo L.; Polack, Fernando Pedro; Talarico, Laura Beatriz; C1q modulation of antibody-dependent enhancement of dengue virus infection in human myeloid cell lines is dependent on cell type and antibody specificity; Elsevier Science; Microbes and Infection; 2024; 6-2024; 1-9
dc.identifier.issn
1286-4579
dc.identifier.uri
http://hdl.handle.net/11336/242365
dc.description.abstract
Antibody dependent enhancement (ADE) of dengue virus (DENV) infection is one of the mechanisms contributing to increased severity during heterotypic, secondary infection. The complement protein C1q has been shown to reduce the magnitude of ADE in vitro. Therefore, we investigated the mechanisms of C1q modulation of ADE, focusing on processes of viral entry.Using a model of ADE of DENV-1 infection in human myeloid cell lines in the presence of monoclonal antibodies, 4G2 and 2H2, we found that C1q produced nearly a 40-fold reduction of ADE of DENV-1 in K562 cells, but had no effect in U937 cells. In K562 cells, C1q reduced adsorption of DENV-1/4G2 and exerted a dual inhibitory effect on adsorption and internalization of DENV-1/2H2. Distinct endocytic pathways in the presence of antibody corresponded to conditions where C1q produced a differential action. Also, C1q did not affect the intrinsic cell response mediated by Fc-gammaR in human myeloid cells. The modulation of ADE of DENV-1 by C1q is dependent on the Fc-gammaR expressed on immune cells and the specificity of the antibody comprising the immune complex. Understanding protective and pathogenic mechanisms in the humoral response to DENV infections is crucial for the successful design of antivirals and vaccines.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
COMPLEMENT
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CIQ
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ANTIBODY-DEPENDENT ENHANCEMENT
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FLAVIVIRUS
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DENGUE VIRUS
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IMMUNOPATHOGENESIS
dc.subject.classification
Enfermedades Infecciosas
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Ciencias de la Salud
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CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
C1q modulation of antibody-dependent enhancement of dengue virus infection in human myeloid cell lines is dependent on cell type and antibody specificity
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2024-08-09T09:59:02Z
dc.journal.volume
2024
dc.journal.pagination
1-9
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Byrne, Alana Brooke. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Gobierno de la Ciudad Autónoma de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Departamento de Medicina.; Argentina. Fundación para la Investigación en Infectología Infantil; Argentina
dc.description.fil
Fil: Bonnin, Florencia Agustina. Gobierno de la Ciudad Autónoma de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Departamento de Medicina.; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
dc.description.fil
Fil: López, Eduardo L.. Gobierno de la Ciudad Autónoma de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Departamento de Medicina.; Argentina
dc.description.fil
Fil: Polack, Fernando Pedro. Fundación para la Investigación en Infectología Infantil; Argentina
dc.description.fil
Fil: Talarico, Laura Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Gobierno de la Ciudad Autónoma de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Departamento de Medicina.; Argentina. Fundación para la Investigación en Infectología Infantil; Argentina
dc.journal.title
Microbes and Infection
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S128645792400114X
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1016/j.micinf.2024.105378
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