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dc.contributor.author
Byrne, Alana Brooke  
dc.contributor.author
Bonnin, Florencia Agustina  
dc.contributor.author
López, Eduardo L.  
dc.contributor.author
Polack, Fernando Pedro  
dc.contributor.author
Talarico, Laura Beatriz  
dc.date.available
2024-08-13T11:39:35Z  
dc.date.issued
2024-06  
dc.identifier.citation
Byrne, Alana Brooke; Bonnin, Florencia Agustina; López, Eduardo L.; Polack, Fernando Pedro; Talarico, Laura Beatriz; C1q modulation of antibody-dependent enhancement of dengue virus infection in human myeloid cell lines is dependent on cell type and antibody specificity; Elsevier Science; Microbes and Infection; 2024; 6-2024; 1-9  
dc.identifier.issn
1286-4579  
dc.identifier.uri
http://hdl.handle.net/11336/242365  
dc.description.abstract
Antibody dependent enhancement (ADE) of dengue virus (DENV) infection is one of the mechanisms contributing to increased severity during heterotypic, secondary infection. The complement protein C1q has been shown to reduce the magnitude of ADE in vitro. Therefore, we investigated the mechanisms of C1q modulation of ADE, focusing on processes of viral entry.Using a model of ADE of DENV-1 infection in human myeloid cell lines in the presence of monoclonal antibodies, 4G2 and 2H2, we found that C1q produced nearly a 40-fold reduction of ADE of DENV-1 in K562 cells, but had no effect in U937 cells. In K562 cells, C1q reduced adsorption of DENV-1/4G2 and exerted a dual inhibitory effect on adsorption and internalization of DENV-1/2H2. Distinct endocytic pathways in the presence of antibody corresponded to conditions where C1q produced a differential action. Also, C1q did not affect the intrinsic cell response mediated by Fc-gammaR in human myeloid cells. The modulation of ADE of DENV-1 by C1q is dependent on the Fc-gammaR expressed on immune cells and the specificity of the antibody comprising the immune complex. Understanding protective and pathogenic mechanisms in the humoral response to DENV infections is crucial for the successful design of antivirals and vaccines.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Elsevier Science  
dc.rights
info:eu-repo/semantics/restrictedAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
COMPLEMENT  
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CIQ  
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ANTIBODY-DEPENDENT ENHANCEMENT  
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FLAVIVIRUS  
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DENGUE VIRUS  
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IMMUNOPATHOGENESIS  
dc.subject.classification
Enfermedades Infecciosas  
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Ciencias de la Salud  
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CIENCIAS MÉDICAS Y DE LA SALUD  
dc.title
C1q modulation of antibody-dependent enhancement of dengue virus infection in human myeloid cell lines is dependent on cell type and antibody specificity  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2024-08-09T09:59:02Z  
dc.journal.volume
2024  
dc.journal.pagination
1-9  
dc.journal.pais
Países Bajos  
dc.journal.ciudad
Amsterdam  
dc.description.fil
Fil: Byrne, Alana Brooke. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Gobierno de la Ciudad Autónoma de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Departamento de Medicina.; Argentina. Fundación para la Investigación en Infectología Infantil; Argentina  
dc.description.fil
Fil: Bonnin, Florencia Agustina. Gobierno de la Ciudad Autónoma de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Departamento de Medicina.; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina  
dc.description.fil
Fil: López, Eduardo L.. Gobierno de la Ciudad Autónoma de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Departamento de Medicina.; Argentina  
dc.description.fil
Fil: Polack, Fernando Pedro. Fundación para la Investigación en Infectología Infantil; Argentina  
dc.description.fil
Fil: Talarico, Laura Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Gobierno de la Ciudad Autónoma de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Departamento de Medicina.; Argentina. Fundación para la Investigación en Infectología Infantil; Argentina  
dc.journal.title
Microbes and Infection  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S128645792400114X  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1016/j.micinf.2024.105378