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dc.contributor.author
Palstra, Arjan P.
dc.contributor.author
Beltran, Sergi
dc.contributor.author
Burgerhout, Erik
dc.contributor.author
Brittijn, Sebastiaan A.
dc.contributor.author
Magnoni, Leonardo Julián
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dc.contributor.author
Henkel, Christiaan V.
dc.contributor.author
Jansen, Hans J.
dc.contributor.author
Van Den Thillart, Guido E. E. J. M.
dc.contributor.author
Spaink, Herman P.
dc.contributor.author
Planas, Josep V.
dc.date.available
2015-10-08T19:02:13Z
dc.date.issued
2013-01-08
dc.identifier.citation
Palstra, Arjan P.; Beltran, Sergi; Burgerhout, Erik; Brittijn, Sebastiaan A.; Magnoni, Leonardo Julián; et al.; Deep RNA Sequencing of the Skeletal Muscle Transcriptome in Swimming Fish; Public Library of Science; Plos One; 8; 1; 8-1-2013; 1-14
dc.identifier.issn
1932-6203
dc.identifier.uri
http://hdl.handle.net/11336/2422
dc.description.abstract
Deep RNA sequencing (RNA-seq) was performed to provide an in-depth view of the transcriptome of red and white skeletal muscle of exercised and non-exercised rainbow trout (Oncorhynchus mykiss) with the specific objective to identify expressed genes and quantify the transcriptomic effects of swimming-induced exercise. Pubertal autumn-spawning seawater-raised female rainbow trout were rested (n = 10) or swum (n = 10) for 1176 km at 0.75 body-lengths per second in a 6,000-L swimflume under reproductive conditions for 40 days. Red and white muscle RNA of exercised and non-exercised fish (4 lanes) was sequenced and resulted in 15–17 million reads per lane that, after de novo assembly, yielded 149,159 red and 118,572 white muscle contigs. Most contigs were annotated using an iterative homology search strategy against salmonid ESTs, the zebrafish Danio rerio genome and general Metazoan genes. When selecting for large contigs (.500 nucleotides), a number of novel rainbow trout gene sequences were identified in this study: 1,085 and 1,228 novel gene sequences for red and white muscle, respectively, which included a number of important molecules for skeletal muscle function. Transcriptomic analysis revealed that sustained swimming increased transcriptional activity in skeletal muscle and specifically an upregulation of genes involved in muscle growth and developmental processes in white muscle. The unique collection of transcripts will contribute to our understanding of red and white muscle physiology, specifically during the long-term reproductive migration of salmonids.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Public Library of Science
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/
dc.subject
Skeletal Muscle Transcriptome
dc.subject
Endurance Exercise
dc.subject
Fish Physiology
dc.subject
Rna Sequencing
dc.subject.classification
Genética y Herencia
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.subject.classification
Biología Marina, Limnología
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Deep RNA Sequencing of the Skeletal Muscle Transcriptome in Swimming Fish
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-03-30 10:35:44.97925-03
dc.journal.volume
8
dc.journal.number
1
dc.journal.pagination
1-14
dc.journal.pais
Estados Unidos
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dc.journal.ciudad
San Francisco
dc.description.fil
Fil: Palstra, Arjan P.. Universidad de Barcelona. Facultad de Biología; España;
dc.description.fil
Fil: Beltran, Sergi. Universidad de Barcelona; España
dc.description.fil
Fil: Burgerhout, Erik. No especifíca;
dc.description.fil
Fil: Brittijn, Sebastiaan A.. No especifíca;
dc.description.fil
Fil: Magnoni, Leonardo Julián. Universidad de Barcelona; España. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Henkel, Christiaan V.. No especifíca;
dc.description.fil
Fil: Jansen, Hans J.. No especifíca;
dc.description.fil
Fil: Van Den Thillart, Guido E. E. J. M.. No especifíca;
dc.description.fil
Fil: Spaink, Herman P.. No especifíca;
dc.description.fil
Fil: Planas, Josep V.. Universidad de Barcelona; España
dc.journal.title
Plos One
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/DOI:10.1371/journal.pone.0053171
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3540090/
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0053171
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