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dc.contributor.author
Prego, Alejo Facundo
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dc.contributor.author
Alonso, Guillermo Daniel
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dc.date.available
2024-08-09T09:34:13Z
dc.date.issued
2023
dc.identifier.citation
Axenic culture of Trypanosoma cruzi amastigotes as a model for differential metabolic characteristics in the intracellular parasite form; XXXIV Reunión anual de la Sociedad Argentina de Protozoología; La Plata; Argentina; 2023; 79-80
dc.identifier.issn
2953-5751
dc.identifier.uri
http://hdl.handle.net/11336/242147
dc.description.abstract
Trypanosoma cruzi has a complex life cycle, alternating between an insect vector and the human host, with three well-defined stages: epimastigotes, trypomastigotes, and amastigotes. The stages present in humans are bloodstream trypomastigotes, which is the infective but non-replicative form, and amastigotes, the intracellular replicative form, which are the focus of clinical treatments. Currently, there are two drugs used for treatment: benznidazole (Bz) and nifurtimox. These drugs must be metabolized by the parasite, so they can have an effect, and have a high degree of toxicity to the patient, often leading to treatment abandonment. Recently, the presence of dormant amastigotes within infected cells was described, and they are considered one of the possible causes of therapeutic failure due to their low or null metabolic activity, being able to resist Bz treatment, redifferentiate into trypomastigotes, and reinfect, causing disease relapse. Previous studies described a strategy to differentiate trypomastigotes into amastigotes axenically, using an acidic medium, and maintaining them in this state for a short period (10 days). In this study, we describe a new formulation that allows doubling the cultivation time of axenic amastigotes while preserving their ability to differentiate into trypomastigotes and maintaining their infective potential, similar behavior as dormants. Additionally, we observed signs of a more active metabolism in amastigotes obtained from infected Vero cell cultures, as opposed to those obtained through in vitro differentiation. Therefore, it is necessary to expand studies to determine whether using axenic amastigotes as a model for cultured amastigotes is equivalent or if they would exhibit reduced metabolic activity, making them more similar to dormants.
dc.format
application/pdf
dc.language.iso
spa
dc.publisher
Sociedad Argentina de Protozoología
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
DIFERENCIACIÓN CELULAR
dc.subject
AMASTIGOGÉNESIS
dc.subject
METABOLISMO
dc.subject
ENFERMEDAD DE CHAGAS
dc.subject.classification
Bioquímica y Biología Molecular
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Axenic culture of Trypanosoma cruzi amastigotes as a model for differential metabolic characteristics in the intracellular parasite form
dc.type
info:eu-repo/semantics/publishedVersion
dc.type
info:eu-repo/semantics/conferenceObject
dc.type
info:ar-repo/semantics/documento de conferencia
dc.date.updated
2024-07-26T13:25:27Z
dc.journal.volume
2
dc.journal.pagination
79-80
dc.journal.pais
Argentina
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dc.journal.ciudad
Ciudad Autónoma de Buenos Aires
dc.description.fil
Fil: Prego, Alejo Facundo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
dc.description.fil
Fil: Alonso, Guillermo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://protozoologia.org.ar/wp-content/uploads/PARASITUS-Volumen-2-2023-ISSN-2953-5751.pdf
dc.conicet.rol
Autor
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dc.conicet.rol
Autor
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dc.coverage
Nacional
dc.type.subtype
Reunión
dc.description.nombreEvento
XXXIV Reunión anual de la Sociedad Argentina de Protozoología
dc.date.evento
2023-11-01
dc.description.ciudadEvento
La Plata
dc.description.paisEvento
Argentina
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dc.type.publicacion
Journal
dc.description.institucionOrganizadora
Sociedad Argentina de Protozoología
dc.source.revista
Parasitus
dc.date.eventoHasta
2023-11-03
dc.type
Reunión
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