Artículo
Different Mitogen-Mediated Beta-Adrenergic Receptor Modulation in Murine T Lymphocytes Depending on the Thyroid Status
Klecha, Alicia Juana
; Barreiro Arcos, María Laura
; Genaro, Ana Maria
; Gorelik, Gabriela Judith
; Silberman, Dafne Magalí
; Caro, Ricardo; Cremaschi, Graciela Alicia
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Fecha de publicación:
12/2005
Editorial:
Karger
Revista:
NeuroImmunoModulation
ISSN:
1021-7401
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
OBJECTIVE: The aim of this work was to analyze beta-adrenergic receptor (betaAR) regulation of T-lymphocyte proliferation in mice according to different thyroid hormone statuses. METHODS: T cells from eu-, hypo- (by propylthiouracil treatment) and hyperthyroid (by thyroxine, T4 administration) mice were purified and specific radioligand binding assays were performed. The effects of the beta-agonist isoproterenol (ISO) on intracellular levels of cyclic AMP (cAMP) were determined. Mitogen-induced T-cell proliferation was measured by [(3)H]-thymidine incorporation. Finally, protein kinase C (PKC) activity in cytosol and membrane fractions were determined using radiolabelled enzymatic substrates. RESULTS: Adecrease or a non-significant increase in betaAR number was found on T lymphocytes from hypo- and hyperthyroid mice compared to euthyroid controls. ISO stimulation of cAMP levels was lower in hypothyroid and higher in hyperthyroid T lymphocytes compared to controls. T-selective mitogen-induced proliferation was increased in T4-treated animals, but decreased in hypothyroid mice. During the peak of proliferation, downregulation of betaAR was observed in all animals. However, a higher or a lower decrease was observed in hyper- and hypothyroid T cells, respectively. In parallel, a higher translocation of PKC activity was observed in hyperthyroid cells, and a lower one was found in hypothyroid lymphocytes with respect to controls. CONCLUSIONS: These results indicate that intracellular signals triggered by mitogen activation, namely PKC, would be related to differential betaAR downregulation in T lymphocytes depending on the thyroid hormone status, contributing to the distinct proliferative responses found in hypo- or hyperthyroidism compared to the euthyroid state.
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Colecciones
Articulos(CEFYBO)
Articulos de CENTRO DE ESTUDIOS FARMACOLOGICOS Y BOTANICOS
Articulos de CENTRO DE ESTUDIOS FARMACOLOGICOS Y BOTANICOS
Articulos(OCA HOUSSAY)
Articulos de OFICINA DE COORDINACION ADMINISTRATIVA HOUSSAY
Articulos de OFICINA DE COORDINACION ADMINISTRATIVA HOUSSAY
Articulos(SEDE CENTRAL)
Articulos de SEDE CENTRAL
Articulos de SEDE CENTRAL
Citación
Klecha, Alicia Juana; Barreiro Arcos, María Laura; Genaro, Ana Maria; Gorelik, Gabriela Judith; Silberman, Dafne Magalí; et al.; Different Mitogen-Mediated Beta-Adrenergic Receptor Modulation in Murine T Lymphocytes Depending on the Thyroid Status; Karger; NeuroImmunoModulation; 12; 2; 12-2005; 92-99
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