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dc.contributor.author
Rivero, Mariana Alejandra
dc.contributor.author
Passucci, Juan Antonio
dc.contributor.author
Rodriguez, Edgardo Mario
dc.contributor.author
Parma, Alberto Ernesto
dc.date.available
2024-08-07T13:03:23Z
dc.date.issued
2010-03
dc.identifier.citation
Rivero, Mariana Alejandra; Passucci, Juan Antonio; Rodriguez, Edgardo Mario; Parma, Alberto Ernesto; Role and clinical course of verotoxigenic Escherichia coli infections in childhood acute diarrhoea in Argentina; Society for General Microbiology; Journal of Medical Microbiology; 59; 3; 3-2010; 345-352
dc.identifier.issn
0022-2615
dc.identifier.uri
http://hdl.handle.net/11336/242002
dc.description.abstract
The aim of the study was to investigate the role and clinical course of verotoxigenic Escherichia coli (VTEC) infections in children with acute diarrhoea from Argentina, the country with the highest worldwide incidence of haemolytic uraemic syndrome (HUS). To accomplish our objective, 437 samples from children up to 6 years old with acute diarrhoea were collected and processed. More than 60% of the children studied presented watery or mucous diarrhoea without blood, and in 25.2% of the cases the samples were with blood. In a first screening, a multiplex PCR was performed to detect the presence of vt1, vt2, eae, ehxA, saa virulence genes Then, the strains were isolated and analyzed in order to characterize their serotypes, virulence genes, antibiotic susceptibility profiles and verotoxin (VT) production. Forty four of the 437 samples (10.06%) were positive to VTEC virulence genes. VTEC-infected patients presented different types of diarrhoea (27.27% belonged to non-bloody type). Several serotypes and virulence genotypes were found. Isolates belonged to the serotypes O157:H7, O145:H-, O26:H11, O121:H19, O111:H2, and O118:H2. HUS developed in 16 (36.4%) patients corresponding to positive samples. All the VTEC isolates produced a cytopathic effect on Vero cells monolayer, confirming the ability for VT expression. Despite most strains were sensitive to all antimicrobials studied a positive association between clinical progression to HUS and antibiotic therapy, both for the total of patients studied, as well as for the VTEC-positive group was observed. In conclusion, the data obtained in this study increase the knowledge on the role and clinical course of VTEC infection in childhood acute diarrhoea beyond bloody diarrhea, and might be considered for the prevention, diagnosis and management of this disease. It is possible that the optimal approach for VTEC diagnosis could be through searching by multiplex PCR for the presence of vt1, vt2, eae and ehxA genes.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Society for General Microbiology
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
VEROTOXIGENIC
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E COLI
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CLINICAL COURSE
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ARGENTINA
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Enfermedades Infecciosas
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Ciencias de la Salud
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CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Role and clinical course of verotoxigenic Escherichia coli infections in childhood acute diarrhoea in Argentina
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2024-08-06T14:39:02Z
dc.journal.volume
59
dc.journal.number
3
dc.journal.pagination
345-352
dc.journal.pais
Reino Unido
dc.journal.ciudad
Londres
dc.description.fil
Fil: Rivero, Mariana Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva; Argentina
dc.description.fil
Fil: Passucci, Juan Antonio. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva; Argentina
dc.description.fil
Fil: Rodriguez, Edgardo Mario. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva; Argentina
dc.description.fil
Fil: Parma, Alberto Ernesto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva; Argentina
dc.journal.title
Journal of Medical Microbiology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.015560-0
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1099/jmm.0.015560-0
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