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dc.contributor.author
Pena, Liliana Beatriz
dc.contributor.author
Tomaro, Maria Lujan
dc.contributor.author
Gallego, Susana Mabel
dc.date.available
2024-08-07T12:02:49Z
dc.date.issued
2006-12
dc.identifier.citation
Pena, Liliana Beatriz; Tomaro, Maria Lujan; Gallego, Susana Mabel; Effect of different metals on protease activity in sunflower cotyledons; Pontificia Universidad Católica de Valparaíso; Electronic Journal of Biotechnology; 9; 3; 12-2006; 258-262
dc.identifier.issn
0717-3458
dc.identifier.uri
http://hdl.handle.net/11336/241980
dc.description.abstract
Proteases are crucial for living cells and play a role in plant cell adaptation to environmental conditions. Oxidative stress produced oxidized proteins which are selectively degraded by proteases. To understand the role of proteolysis in response to metal stress, sunflower plants (a plant suitable for phytoremediation) were treated with 100 μM of CdCl2, CuCl2, AlCl3, CoCl2,PbCl2, CrCl3, NiCl2, HgCl2 or ZnCl2. Changes in protease activity, gelatinase profile and protein oxidation were examined in sunflower cotyledons. Our results indicate that this tissue has mainly acid proteases belonging to different classes. Although all metals (except Zn) increased protein oxidation (62, 57, 112, 74, 74, 68, 64 and 40% for Pb, Al, Ni, Cd, Hg, Co, Cr andCu over the control), they altered proteolysis in different ways. Pb, Al and Ni treatment decreased protease activity 22, 28 and 30% respect to control while Cd and Hg increased this activity in 23 and 27%. In Zn, Cu and Co treatments protease activity remained similar to control treatment. These results indicate that different proteases are involved in plant defence againstmetal toxicity. However, the identification of specific oxidized proteins involved in this process and the metal effect on class specific proteases should provide greater information.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Pontificia Universidad Católica de Valparaíso
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject
PROTEASE
dc.subject
HEAVY METALS
dc.subject.classification
Otras Ciencias Químicas
dc.subject.classification
Ciencias Químicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Effect of different metals on protease activity in sunflower cotyledons
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2024-08-05T13:49:57Z
dc.journal.volume
9
dc.journal.number
3
dc.journal.pagination
258-262
dc.journal.pais
Chile
dc.description.fil
Fil: Pena, Liliana Beatriz. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica. Cátedra de Química Biológica Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
dc.description.fil
Fil: Tomaro, Maria Lujan. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica. Cátedra de Química Biológica Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Gallego, Susana Mabel. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica. Cátedra de Química Biológica Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
dc.journal.title
Electronic Journal of Biotechnology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.ejbiotechnology.info/index.php/ejbiotechnology/article/view/v9n3-18
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