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dc.contributor.author
Peretti, Leandro Ezequiel
dc.contributor.author
Gonzalez, Veronica Doris Guadalupe
dc.contributor.author
Costa, Juan Gabriel
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Marcipar, Ivan Sergio
dc.contributor.author
Gugliotta, Luis Marcelino
dc.date.available
2017-09-13T19:49:28Z
dc.date.issued
2016-08
dc.identifier.citation
Peretti, Leandro Ezequiel; Gonzalez, Veronica Doris Guadalupe; Costa, Juan Gabriel; Marcipar, Ivan Sergio; Gugliotta, Luis Marcelino; Synthesis and Characterization of Latex-protein Complexes from Different Antigens of Toxoplasma gondii for Immunoagglutination Assays; Taylor & Francis; International Journal Of Polymeric Materials; 65; 18; 8-2016; 938-946
dc.identifier.issn
0091-4037
dc.identifier.uri
http://hdl.handle.net/11336/24185
dc.description.abstract
The acute phase recombinant protein of Toxoplasma gondii P22Ag was expressed and purified and thehomogenate of the parasite was obtained from an infected mouse. These antigens were used to producelatex-protein complexes (LPC) through physical adsorption and chemical coupling onto different latexes,with the aim of producing immunoagglutination (IA) reagents able to detect recently acquiredtoxoplasmosis. Polystyrene and ?core-shell? latexes where employed, exhibiting varied particle size,functionality (carboxyl or epoxy), and charge density. In sensitization experiments for producing LPC, therecombinant protein showed better coupling efficiency onto the particles surface than the homogenateand this could be explained by the complex mixture of the homogenate, which includes a large numberof proteins of different molecular mass, isoelectric points, and hydrophobicity. The synthesized LPC wereemployed in IA assays. To this effect, the agglutination reaction was followed by measuring the changesin the optical absorbance by turbidimetry. Experiments against control sera were performed to evaluatethe performance of various LPC and it was observed that the IA test based on P22Ag and thecarboxylated latex of 350 nm of particle diameter allowed a good discrimination between acute sera andchronic/negative ones. The proposed test is cheap, rapid, and easy to implement.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Taylor & Francis
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Acute Phase Recombinant Protein
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Immunoagglutination
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Latex Protein Complex
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Toxoplasmosis
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Compuestos
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Ingeniería de los Materiales
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INGENIERÍAS Y TECNOLOGÍAS
dc.title
Synthesis and Characterization of Latex-protein Complexes from Different Antigens of Toxoplasma gondii for Immunoagglutination Assays
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-09-04T15:08:49Z
dc.journal.volume
65
dc.journal.number
18
dc.journal.pagination
938-946
dc.journal.pais
Reino Unido
dc.journal.ciudad
Londres
dc.description.fil
Fil: Peretti, Leandro Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
dc.description.fil
Fil: Gonzalez, Veronica Doris Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
dc.description.fil
Fil: Costa, Juan Gabriel. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Marcipar, Ivan Sergio. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Gugliotta, Luis Marcelino. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
dc.journal.title
International Journal Of Polymeric Materials
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.tandfonline.com/doi/full/10.1080/00914037.2016.1180611
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1080/00914037.2016.1180611
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