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dc.contributor.author
Marchiando, Nancy Cristina
dc.contributor.author
Zon, María Alicia
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dc.contributor.author
Fernandez, Hector
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dc.date.available
2024-08-06T11:41:47Z
dc.date.issued
2005-09
dc.identifier.citation
Marchiando, Nancy Cristina; Zon, María Alicia; Fernandez, Hector; Determination of cercosporin (CER) phytotoxin isolated from infected peanut leaves by using adsorptive stripping square wave voltammetry; Elsevier Science; Analytica Chimica Acta; 550; 1-2; 9-2005; 199-203
dc.identifier.issn
0003-2670
dc.identifier.uri
http://hdl.handle.net/11336/241841
dc.description.abstract
The adsorptive accumulation of cercosporin (CER) phytotoxin on glassy carbon (GC) electrodes from 1 M HClO4 aqueous solutions is used to detect and quantify the phytotoxin isolated from infected leaf tissue collected from field-grown peanut in five locations in southern regions of the Provinces of Córdoba and San Luis, Argentina. The adsorptive stripping square wave voltammetry (ASSWV) is employed to perform the quantitative determination of CER in naturally infected extracts. Thin layer chromatography (TLC) and HPLC were used to verify the presence of CER in extracts. Values of Rf = 0.23 and Rf = 0.15 were determined for CER by TLC when extracts were dissolved in ethyl acetate (EA) and acetone (Ac), respectively, while a retention time of 6.13 min (Ac) was determined by HPLC. The recovery percentage of CER using ASSWV was determined by employing healthy peanut leaf tissue spiked with standard CER. The application of standard addition method was used to determine the recovery percentage as well as the CER contamination level in infected extracts. A recovery percentage of 93.27% was obtained with a relative standard deviation of 21.8 % for a solution concentration of 1.04 x 10-6 mol dm-3 (n=11). The limit of detection for a signal to noise ratio was about 6 ppb and the quantitation limit was about 50 ppb. The pondered relative standard deviation of 9.5 % was calculated for the ASSWV method. Results found for CER contamination level in extracts through the electroanalytical method here proposed are in reasonable agreement with those values determined by using HPLC measurements.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
CERCOSPORIN
dc.subject
PEANUT LEAVES
dc.subject
ADSORPTIVE STRIPPING VOLTAMMETRY
dc.subject
SQUARE WAVE VOLTAMMETRY
dc.subject.classification
Química Analítica
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dc.subject.classification
Ciencias Químicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Determination of cercosporin (CER) phytotoxin isolated from infected peanut leaves by using adsorptive stripping square wave voltammetry
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2024-08-06T11:17:35Z
dc.journal.volume
550
dc.journal.number
1-2
dc.journal.pagination
199-203
dc.journal.pais
Países Bajos
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dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Marchiando, Nancy Cristina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina
dc.description.fil
Fil: Zon, María Alicia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
dc.description.fil
Fil: Fernandez, Hector. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
dc.journal.title
Analytica Chimica Acta
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S000326700501069X
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.aca.2005.06.054
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