Mostrar el registro sencillo del ítem

dc.contributor.author
Marchiando, Nancy Cristina  
dc.contributor.author
Zon, María Alicia  
dc.contributor.author
Fernandez, Hector  
dc.date.available
2024-08-06T11:41:34Z  
dc.date.issued
2005-09  
dc.identifier.citation
Marchiando, Nancy Cristina; Zon, María Alicia; Fernandez, Hector; Determination of cercosporin (CER) phytotoxin isolated from infected peanut leaves by using adsorptive stripping square wave voltammetry; Elsevier Science; Analytica Chimica Acta; 550; 1-2; 9-2005; 199-203  
dc.identifier.issn
0003-2670  
dc.identifier.uri
http://hdl.handle.net/11336/241839  
dc.description.abstract
The adsorptive accumulation of cercosporin (CER) phytotoxin on glassy carbon (GC) electrodes from 1 M HClO4 aqueous solutions is used to detect and quantify the phytotoxin isolated from infected leaf tissue collected from field-grown peanut in five locations in southern regions of the Provinces of Cordoba and San Luis, Argentina. The adsorptive stripping square wave voltammetry (ASSWV) is employed to ´ perform the quantitative determination of CER in naturally infected extracts. Thin layer chromatography (TLC) and HPLC were used to verify the presence of CER in extracts. Values of Rf = 0.23 and Rf = 0.15 were determined for CER by TLC when extracts were dissolved in ethyl acetate (EA) and acetone (Ac), respectively, while a retention time of 6.13 min (Ac) was determined by HPLC. The recovery percentage of CER using ASSWV was determined by employing healthy peanut leaf tissue spiked with standard CER. The application of standard addition method was used to determine the recovery percentage as well as the CER contamination level in infected extracts. A recovery percentage of 93.27% was obtained with a relative standard deviation of 21.8% for a solution concentration of 1.04 × 10−6 mol dm−3 (n = 11). The limit of detection for a signal to noise ratio of 3:1 was about 6 ppb and the quantitation limit was about 50 ppb. The pondered relative standard deviation of 9.5% was calculated for the ASSWV method. Results found for CER contamination level in extracts through the electroanalytical method here proposed are in reasonable agreement with those values determined by using HPLC measurements.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Elsevier Science  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
Cercosporin  
dc.subject
Phytotoxins  
dc.subject
Peanut  
dc.subject
Square wave voltammetry  
dc.subject
Adsorptive accumulation  
dc.subject.classification
Físico-Química, Ciencia de los Polímeros, Electroquímica  
dc.subject.classification
Ciencias Químicas  
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS  
dc.title
Determination of cercosporin (CER) phytotoxin isolated from infected peanut leaves by using adsorptive stripping square wave voltammetry  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2024-08-06T11:17:59Z  
dc.journal.volume
550  
dc.journal.number
1-2  
dc.journal.pagination
199-203  
dc.journal.pais
Países Bajos  
dc.journal.ciudad
Amsterdam  
dc.description.fil
Fil: Marchiando, Nancy Cristina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina  
dc.description.fil
Fil: Zon, María Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina  
dc.description.fil
Fil: Fernandez, Hector. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina  
dc.journal.title
Analytica Chimica Acta  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S000326700501069X  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.aca.2005.06.054