Artículo
Estrogens sensitize anterior pituitary gland to apoptosis
Pisera, Daniel Alberto
; Candolfi, Marianela
; Navarra, S.; Ferraris, Maria Jimena
; Zaldivar, Verónica
; Jaita, Gabriela Alejandra
; Castro, M. G.; Seilicovich, Adriana
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Fecha de publicación:
10/2004
Editorial:
American Physiological Society
Revista:
American Journal Of Physiology-endocrinology And Metabolism
ISSN:
0193-1849
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Tissue homeostasis results from a balance between cell proliferation and cell death by apoptosis. Estradiol affects proliferation as well as apoptosis in hormone-dependent tissues. In the present study, we investigated the apoptotic response of the anterior pituitary gland to lipopolysaccharide (LPS) in cycling female rats, and the influence of estradiol in this response in ovariectomized (OVX) rats. The OVX rats were chronically estrogenized with implanted Silastic capsules containing 1 mg of 17beta-estradiol (E2). Cycling or OVX and E2-treated rats were injected with LPS (250 microg/rat ip). Apoptosis was determined by the terminal deoxynucleotidyl-mediated dUTP nick-end labeling (TUNEL) method in sections of the anterior pituitary gland and spleen. Chronic estrogenization induced apoptosis in the anterior pituitary gland. Acute endotoxemia triggered apoptosis of cells in the anterior pituitary gland of E2-treated rats but not of OVX rats. No differences were observed in the apoptotic response to LPS in spleen between OVX and E2-treated rats. The apoptotic response of the anterior pituitary to LPS was variable along the estrous cycle, being higher at proestrus than at estrus or diestrus I. Approximately 75% of the apoptotic cells were identified as lactotropes by immunofluorescence. In conclusion, our results indicate that estradiol induces apoptosis and enables the proapoptotic action of LPS in the anterior pituitary gland. Also, our study suggests that estrogens may be involved in anterior pituitary cell renewal during the estrous cycle, sensitizing lactotropes to proapoptotic stimuli.
Palabras clave:
ESTRADIOL
,
ANTERIOR PITUITARY
,
LPS
,
APOPTOSIS
Archivos asociados
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Identificadores
Colecciones
Articulos(INBIOMED)
Articulos de INSTITUTO DE INVESTIGACIONES BIOMEDICAS
Articulos de INSTITUTO DE INVESTIGACIONES BIOMEDICAS
Articulos(OCA HOUSSAY)
Articulos de OFICINA DE COORDINACION ADMINISTRATIVA HOUSSAY
Articulos de OFICINA DE COORDINACION ADMINISTRATIVA HOUSSAY
Citación
Pisera, Daniel Alberto; Candolfi, Marianela; Navarra, S.; Ferraris, Maria Jimena; Zaldivar, Verónica; et al.; Estrogens sensitize anterior pituitary gland to apoptosis; American Physiological Society; American Journal Of Physiology-endocrinology And Metabolism; 287; 4; 10-2004; 767-771
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