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dc.contributor.author
Ozu, Marcelo
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dc.contributor.author
Toriano, Roxana Mabel
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dc.contributor.author
Capurro, Claudia Graciela
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dc.contributor.author
Parisi, Mario Nestor
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dc.date.available
2024-08-05T14:42:42Z
dc.date.issued
2005-10
dc.identifier.citation
Ozu, Marcelo; Toriano, Roxana Mabel; Capurro, Claudia Graciela; Parisi, Mario Nestor; Electrical parameters and water permeability properties in monolayers formed by epithelial cells cultured on permeable supports; Associação Brasileira de Divulgação Científica; Brazilian Journal of Medical and Biological Research; 38; 1; 10-2005; 133-140
dc.identifier.issn
0100-879X
dc.identifier.uri
http://hdl.handle.net/11336/241734
dc.description.abstract
T84 is an established cell line expressing an enterocyte phenotype whose permeability properties have been widely explored. Osmotic permeability (POSM), hydraulic permeability (PHYDR) and transport associated net water fluxes (JW-transp), as well as short-circuit current (ISC), transepithelial resistance (RT), and potential difference (ÄVT) were measured in T84 monolayers with the following results: POSM 1.3 ± 0.1 cm.s-1 x 10-3; PHYDR 0.27 ± 0.02 cm.s-1; RT 2426 ± 109 Ù.cm2, and ÄVT 1.31 ± 0.38 mV. The effect of 50 ìM 5,6-dichloro-1-ethyl-1,3- dihydro-2H-benzimidazol-2-one (DCEBIO), a “net Cl- secretory agent”, on T84 cells was also studied. We confirm the reported important increase in ISC induced by DCEBIO which was associated here with a modest secretory ÄJW-transp. The present results were compared with those reported using the same experimental approach applied to established cell lines originating from intestinal and renal epithelial cells (Caco-2, LLC-PK1 and RCCD-1). No clear association between PHYDR and RT could be demonstrated and high PHYDR values were observed in an electrically tight epithelium, supporting the view that a “water leaky” barrier is not necessarily an “electrically leaky” one. Furthermore, the modest secretory ÄJW-transp was not consistent with previous results obtained with RCCD-1 cells stimulated with vasopressin (absorptive fluxes) or with T84 cells secreting water under the action of Escherichia coli heat stable enterotoxin. We conclude that, while the presence of aquaporins is necessary to dissipate an external osmotic gradient, coupling between water and ion transport cannot be explained by a simple and common underlying mechanism.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Associação Brasileira de Divulgação Científica
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/
dc.subject
OSMOTIC GRADIENTS
dc.subject
AQUAPORINS
dc.subject
T84 CELLS
dc.subject
WATER ABSORPTION AND SECRETION
dc.subject.classification
Fisiología
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dc.subject.classification
Medicina Básica
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dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
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dc.title
Electrical parameters and water permeability properties in monolayers formed by epithelial cells cultured on permeable supports
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2024-08-05T13:40:19Z
dc.identifier.eissn
1414-431X
dc.journal.volume
38
dc.journal.number
1
dc.journal.pagination
133-140
dc.journal.pais
Brasil
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dc.journal.ciudad
San Pablo
dc.description.fil
Fil: Ozu, Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Biomembranas; Argentina
dc.description.fil
Fil: Toriano, Roxana Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Biomembranas; Argentina
dc.description.fil
Fil: Capurro, Claudia Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Biomembranas; Argentina
dc.description.fil
Fil: Parisi, Mario Nestor. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Biomembranas; Argentina
dc.journal.title
Brazilian Journal of Medical and Biological Research
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.scielo.br/j/bjmbr/a/6dfbyqpNkfDndsp78CWVwTb/?lang=en
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1590/S0100-879X2005000100020
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