Artículo
Expression and Localization of StarD7 in Trophoblast Cells
Angeletti, Sofia Claudia
; Rena, Viviana Celeste del Valle
; Nores, Rodrigo
; Fretes, Ricardo Emilio; Panzetta-Dutari, Graciela Maria del Valle
; Genti de Raimondi, Susana
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Fecha de publicación:
05/2008
Editorial:
W B Saunders Co Ltd
Revista:
Placenta
ISSN:
0143-4004
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
The StAR-related lipid transfer (START) domain is defined as a motif of around 200 amino acids implicated in lipid/sterol binding. In a previous study, we identified the StarD7 transcript encoding one of the 15 family members with START domain present in the human genome. This transcript was found to be overexpressed in choriocarcinoma JEG-3 cells. In addition, we demonstrated that the recombinant StarD7 protein forms stable Gibbs and Langmuir monolayers at the air-buffer interface, showing marked surface activity and interaction with phospholipid monolayers, mainly with phosphatidylserine, cholesterol and phosphatidylglycerol. This study was undertaken to evaluate the expression and localization of StarD7 protein in trophoblastic samples. Here, we show for the first time the presence of StarD7 protein in human trophoblast cells. Western blot assays revealed a unique specific 34 kDa protein in JEG-3 cell line, choriocarcinoma tissue, complete hydatidiform mole, early and normal term placenta. Immunohistochemical data from early and normal term placentas and complete hydatidiform moles showed that this protein is abundant in the syncytiotrophoblasts, mainly at the apical side of the syncytium, with a weak and focal reaction in the cytotrophoblast cells. Furthermore, an increased StarD7 mRNA and protein expression, as well as a change in its sub-cellular localization was observed in in vitro differentiating cytotrophoblast isolated from normal term placenta. Taken together, these findings support and allow future studies to explore the possibility that StarD7 protein mediates transplacental lipid transport and/or is involved in syncytialization.
Palabras clave:
LIPID-TRANSPORT
,
STARD7
,
START DOMAIN
,
TROPHOBLAST
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Identificadores
Colecciones
Articulos(CCT - CORDOBA)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - CORDOBA
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - CORDOBA
Articulos(CIBICI)
Articulos de CENTRO DE INV.EN BIOQUI.CLINICA E INMUNOLOGIA
Articulos de CENTRO DE INV.EN BIOQUI.CLINICA E INMUNOLOGIA
Citación
Angeletti, Sofia Claudia; Rena, Viviana Celeste del Valle; Nores, Rodrigo; Fretes, Ricardo Emilio; Panzetta-Dutari, Graciela Maria del Valle; et al.; Expression and Localization of StarD7 in Trophoblast Cells; W B Saunders Co Ltd; Placenta; 29; 5; 5-2008; 396-404
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