Mostrar el registro sencillo del ítem
dc.date.available
2024-08-02T15:30:25Z
dc.identifier.citation
Videla, Emiliano Ariel; Luna, Agustin; Picco, Romina Paula Andrea; Marin, Raul Hector; Nazar, Franco Nicolas; Caliva, Jorge Martín; (2024): Thymol, alpha tocopherol, and ascorbyl palmitate as a natural feed strategy to modulate immune variables and Heterophil to Lymphocyte ratio in broiler chickens. Consejo Nacional de Investigaciones Científicas y Técnicas. (dataset). http://hdl.handle.net/11336/241615
dc.identifier.uri
http://hdl.handle.net/11336/241615
dc.description.abstract
Immune response observed on broiler chickens fed different natural supplements (BHT, Thymol and a mix of Ascorbyl palmitate and alpha tocopherol
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.title
Thymol, alpha tocopherol, and ascorbyl palmitate as a natural feed strategy to modulate immune variables and Heterophil to Lymphocyte ratio in broiler chickens
dc.type
dataset
dc.date.updated
2024-08-02T15:29:21Z
dc.description.fil
Fil: Videla, Emiliano Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
dc.description.fil
Fil: Luna, Agustin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
dc.description.fil
Fil: Picco, Romina Paula Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
dc.description.fil
Fil: Marin, Raul Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
dc.description.fil
Fil: Nazar, Franco Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
dc.description.fil
Fil: Caliva, Jorge Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
dc.datacite.PublicationYear
2024
dc.datacite.Creator
Videla, Emiliano Ariel
dc.datacite.Creator
Luna, Agustin
dc.datacite.Creator
Picco, Romina Paula Andrea
dc.datacite.Creator
Marin, Raul Hector
dc.datacite.Creator
Nazar, Franco Nicolas
dc.datacite.Creator
Caliva, Jorge Martín
dc.datacite.affiliation
Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas
dc.datacite.affiliation
Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas
dc.datacite.affiliation
Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas
dc.datacite.affiliation
Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas
dc.datacite.affiliation
Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas
dc.datacite.affiliation
Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas
dc.datacite.publisher
Consejo Nacional de Investigaciones Científicas y Técnicas
dc.datacite.subject
Ciencias Veterinarias
dc.datacite.subject
Ciencias Veterinarias
dc.datacite.subject
CIENCIAS AGRÍCOLAS
dc.datacite.date
15/03/2016-15/10/2016
dc.datacite.DateType
Recolectado
dc.datacite.language
spa
dc.datacite.version
1.0
dc.datacite.description
2.1 Animals and Husbandry One-day-old Cobb-500 male broiler chicks with similar weight (43.0 ± 1.0) g were obtained from a local hatchery (INDACOR S.A.), and located in a commercial farm operated by INDACOR S.A. Upon arrival, birds were identified (leg banded for 2 weeks and wing banded after that) and randomly housed in groups of 40 individuals in one of 24 pens measuring 2.4×1.2 m. Water and feed were provided ad libitum throughout the experiment. Appropriate amounts of dietary supplements (see below) were dissolved in soybean oil (normally included in feed’s formulation), sprayed to ground basal diet and mixed until homogenization before extrusion. 2.2 Treatment procedures All birds within each pen were randomly assigned to 1 of 6 dietary treatments. There were 4 replicates (pens) of each dietary treatment. Each replica initially consisted of 40 birds. Dietary treatments were characterized as follows: 1) Basal (no feed supplements added), 2) Promotor (Basal + 6.26 µmol flavomycin/kg feed), 3) BHT (Basal + 1.33 mmol of BHT/kg feed), 4) Prom-BHT (Basal + a mix of 6.26 µmol flavomycin/kg feed and 1.33 mmol of BHT/kg feed), 5) THY (Basal + 1.33 mmoles of THY/kg feed), and 6) TOC-AP (Basal + 0.665 mmoles of TOC + 0.665 mmoles of AP/kg feed). The Basal, Promotor (Flavomycin 8%, Hugestone Enterprise Co., China), BHT (FlukaAG, Buchs SG, Switzerland), and Prom-BHT, were used as controls. THY (SAFCR©, ≥99%, FCC, USA) and TOC-AP (GRINDOX 497 DuPONT, Danisco Argentina) were considered the experimental groups. When birds were 21 days (d) old, stocking density in each pen was randomly reduced to 32 birds per pen. Doses were selected based on previous reports28 and the current promoter and BHT doses used in the farm. All supplements but flavomycin (commercial doses as growth promoter) were chosen to achieve the same final (1.33 mol/kg) concentration. The feeding regimen consisted of a starter (1–10 d), grower (11–26 d), and finisher (27–42 d) diet. The ingredients and chemical composition of the basal diets are detailed elsewhere13. Birds were raised following a standard husbandry program for slaughter weight between 2.5-3.0 kg (Cobb-vantress; Broiler Management Guide 2017). 2.3 Sampling procedures On d 35 of age (one week before slaughtering) blood samples were taken from the left brachial vein of 10 broilers from each of the 24 pens (40 birds in total from each dietary treatment). Blood withdrawal was done with ethylenediaminetetraacetic-acid (EDTA) to avoid blood coagulation; anaesthesia was not used in line with guidance in the National Centre for the Replacement Refinement and Reduction of Animals in Research (www.nc3rs.org.uk/general-principles). The sampling procedure was randomly performed between treatments. One blood drop was used for blood smears, and the rest was centrifuged at 2500 g for 15 minutes to obtain plasma, pellets were discarded. Plasma samples were stored at -80ºC and then used for microagglutination assay to determine humoral response against SRBC. 2.4 Immunological determinations To assess the inflammatory response, the day before blood samples were taken, the peripheral zone of the right-wing web was measured with a digital caliper and then intradermally injected with 0,1 ml of PHA-P (Sigma Chemical, St Louis, USA) solution in phosphate saline buffer (PBS, pH= 7, 1 mg.ml-1) (Vinkler et al., 2010). Twenty-four hours later the same region previously injected was measured to determine the percentage of inflammation, calculated using the following formulae: percentage of inflammation = [(inflammation previous 24hs) / (inflammation post 24hs)] x 100. For the microagglutination assay, a 30 µl aliquot of plasma was used as a pure sample. Another 30 µl aliquot of plasma was serially diluted in 30µl of PBS, across 10 wells of a 96 U well plate. Then, 30 µl of a 2% solution of SRBC were added to each well, except for the blanks. The 11 and the 12 wells were used as blank (serum diluted with PBS only) and negative controls (PBS with SRBC), respectively. Plates were incubated for 45 minutes at 40ºC. Antibody titers were reported as the Log2 of the highest dilution yielding significant agglutination. Blood smears previously made were stained with May-Grünwald Giemsa. Using a light optical microscope (1000x), 100 white cells per smear were counted and differentiated in Heterophils, Basophils, Eosinophils, Monocytes and Lymphocytes. The Heterophil to Lymphocyte ratio was calculated for each individual. H/L ratio was calculated using the following formulae: H/L ratio = (number of heterophils) / (number of lymphocytes) (Nazar et al., 2018). 2.5 Statistical analysis Generalized Linear Mixed Models (GLMM) were used to compare differences between groups on each response variable. According to the data distribution, the percentage of inflammation, titers against SRBC and H/L ratio variables were respectively analyzed using a Gamma, Gamma, and Lognormal function. Dietary treatment was defined as a fixed effect, considering the pen as an experimental unit and as a random effect. DGC test was performed for post-hoc analysis29. A P value < 0.05 was considered to represent significant differences. Statistical analyses were performed through an ‘R’ (The R Foundation for Statistical Computing) user-friendly interface implemented in InfoStat (Di Rienzo et el., 2016).
dc.datacite.DescriptionType
Información Técnica
dc.datacite.FundingReference
22920170100070CO
dc.datacite.FunderName
Consejo Nacional de Investigaciones Científicas y Técnicas
dc.subject.keyword
Immunity
dc.subject.keyword
Welfare
dc.subject.keyword
Poultry
dc.subject.keyword
Supplementation
dc.datacite.resourceTypeGeneral
dataset
dc.conicet.datoinvestigacionid
19390
dc.datacite.awardTitle
Confluencia de las capacidades científicas y biotecnológicas del IIBYT orientadas a la valorización de residuos de la industria alimentaria y a la producción y el bienestar animal.
dc.datacite.geolocation
Córdoba
dc.datacite.formatedDate
2016
Archivos del conjunto de datos
Archivo
Notas de uso
Tamaño