Artículo
Study of two glycosyltransferases related to polysaccharide biosynthesis in Rhodococcus jostii RHA1
Cereijo, Antonela Estefanía
; Ferretti, María Victoria
; Iglesias, Alberto Alvaro
; Álvarez, Héctor Manuel; Asención Diez, Matías Damián
![Icon](/themes/CONICETDigital/images/conicet.png)
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Fecha de publicación:
03/2024
Editorial:
De Gruyter
Revista:
Biological Chemistry
ISSN:
1431-6730
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
The bacterial genus Rhodococcus comprises organisms performing oleaginous behaviors under certain growth conditions and ratio of carbon and nitrogen availability. Rhodococci are outstanding producers of biofuel precursors, where lipid and glycogen metabolisms are closely related. Thus, a better understanding of rhodococcal carbon partitioning requires identifying catalytic steps redirecting sugar moieties to storage molecules. Here, we analyzed two GT4glycosyl-transferases from R. jostii (RjoGlgAb and RjoGlgAc) annotated as α-glucan-α-1,4-glucosyl transferases, putatively involved in glycogen synthesis. Both enzymes were produced in E. coli cells, purified to homogeneity, and kinetically characterized. RjoGlgAb and RjoGlgAc presented the “canonical” glycogen synthase activity and were actives as maltose-1P synthases, although to a different extent. Then, RjoGlgAc is a homologous enzyme to the mycobacterial GlgM, with similar kinetic behavior and glucosyl-donor preference. RjoGlgAc was two orders of magnitude more efficient to glucosylate glucose-1P than glycogen, also using glucosamine-1P as a catalytically efficient aglycon. Instead, RjoGlgAb exhibited both activities with similar kinetic efficiency and preference for short-branched α-1,4-glucans. Curiously, RjoGlgAb presented a super-oligomeric conformation (higher than 15 subunits), representing a novel enzyme with a unique structure-to-function relationship. Kinetic results presented herein constitute a hint to infer on polysaccharides biosynthesis in rhodococci from an enzymological point of view.
Palabras clave:
glycogen
,
glucosamine-1P
,
glucose-1P
,
maltose-1P
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Articulos(IAL)
Articulos de INSTITUTO DE AGROBIOTECNOLOGIA DEL LITORAL
Articulos de INSTITUTO DE AGROBIOTECNOLOGIA DEL LITORAL
Citación
Cereijo, Antonela Estefanía; Ferretti, María Victoria; Iglesias, Alberto Alvaro; Álvarez, Héctor Manuel; Asención Diez, Matías Damián; Study of two glycosyltransferases related to polysaccharide biosynthesis in Rhodococcus jostii RHA1; De Gruyter; Biological Chemistry; 405; 5; 3-2024; 325-340
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