Artículo
Tl(I) and Tl(III) induce reticulum stress in MDCK cells
Morel Gómez, Emanuel Dario
; Casali, Cecilia Irene
; Fernandez, Maria del Carmen
; Verstraeten, Sandra Viviana
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Fecha de publicación:
06/2023
Editorial:
Elsevier Science
Revista:
Environmental Toxicology and Pharmacology
ISSN:
1382-6689
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
The effects of the exposure of proliferating MDCK cells to thallium [Tl(I) or Tl(III)] on cell viability and proliferation were investigated. Although Tl stopped cell proliferation, the viability was > 95%. After 3 h, two autophagy markers (SQSTM-1 expression and LC3β localization) were altered, and at 48 h increased expression of SQSTM-1 (60%) and beclin-1 (50-100%) were found. At 24 h, the expression of endoplasmic reticulum (ER) stress markers ATF-6 and IRE-1 were increased in 100% and 150%, respectively, accompanied by XBP-1 splicing and nuclear translocation. At 48 h, major ultrastructure abnormalities were found, including ER enlargement and cytoplasmic vacuolation which was not prevented by protein synthesis inhibition. Increased PHB (85% and 40% for Tl(I) and Tl(III), respectively) and decreased β-tubulin (45%) expression were found which may be related to the promotion of paraptosis. In summary, Tl(I) and Tl(III) promoted ER stress and probably paraptosis in MDCK cells, impairing their proliferation.
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Articulos(IQUIFIB)
Articulos de INST.DE QUIMICA Y FISICO-QUIMICA BIOLOGICAS "PROF. ALEJANDRO C. PALADINI"
Articulos de INST.DE QUIMICA Y FISICO-QUIMICA BIOLOGICAS "PROF. ALEJANDRO C. PALADINI"
Citación
Morel Gómez, Emanuel Dario; Casali, Cecilia Irene; Fernandez, Maria del Carmen; Verstraeten, Sandra Viviana; Tl(I) and Tl(III) induce reticulum stress in MDCK cells; Elsevier Science; Environmental Toxicology and Pharmacology; 101; 6-2023; 1-14
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