Mostrar el registro sencillo del ítem
dc.contributor.author
Toro Urrego, Nicolas
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.contributor.author
Luaces, Juan Pablo
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.contributor.author
Bordet, Sofía
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.contributor.author
Otero-losada, Matilde Estela
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.contributor.author
Capani, Francisco
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.date.available
2024-07-30T15:47:50Z
dc.date.issued
2024-05
dc.identifier.citation
Toro Urrego, Nicolas; Luaces, Juan Pablo; Bordet, Sofía; Otero-losada, Matilde Estela; Capani, Francisco; Raloxifene Protects Oxygen-glucose-deprived Astrocyte Cells Used To Mimic Hypoxic-ischemic Brain Injury; Multidisciplinary Digital Publishing Institute; Preprints.org; 5-2024; 1-15
dc.identifier.uri
http://hdl.handle.net/11336/241277
dc.description.abstract
Background. Hypoxic-ischemic brain injury is one primary cause of long-term neurologicaldisability, morbidity, and death worldwide. The decrease in blood flow and oxygen concentration leads toinsufficient nutrient supply to the brain, energy depletion, increased free radical generation, and mitochondrialdysfunction. Perinatal asphyxia — the oxygen supply suspension near birth-time — causes hypoxic-ischemicbrain injury and is a major risk factor for neurodevelopmental damage. In pathological scenarios, raloxifene, aselective estrogen receptor modulator, has shown neuroprotective effects. Purpose. To examine whetherraloxifene showed neuroprotection in an oxygen-glucose deprivation/reoxygenation astrocyte cell model.Methods. T98G cells in culture were treated with a glucose-free DMEM medium and incubated at 37ºC in ahypoxia chamber with 1% O2 for 3, 6, 12, and 24 hours. Cultures were supplemented with raloxifene 1, 10, and100 nM during both glucose and oxygen deprivation and reoxygenation periods. Results. Raloxifene 100 nMand 10 nM improved cell survival — 65.34% and 70.56% — respectively compared to the control cell groups.Mitochondrial membrane potential was preserved by 58.9% 10 nM raloxifene and 81.57% 100 nM raloxifenecotreatment. Raloxifene cotreatment reduced superoxide production by 72.72% and peroxide production by57%. Mitochondrial mass was preserved by 47.4%, 75.5%, and 89% in T98G cells exposed to 6-hour oxygenglucosedeprivation followed by 3, 6, and 9 hours of reoxygenation, respectively. Conclusions. Raloxifeneimproved cell survival and mitochondrial membrane potential, and reduced lipid peroxidation and reactiveoxygen species (ROS) production, suggesting a direct effect on mitochondria. Raloxifene protected the oxygenglucose-deprived astrocyte cells used to mimic hypoxic-ischemic brain injury in this study.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Multidisciplinary Digital Publishing Institute
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
RALOXIFENE
dc.subject
NEUROPROTECTION
dc.subject
ASTROCYTES GLUCOSE DEPRIVATION
dc.subject
OXYGEN DEPRIVATION
dc.subject.classification
Neurociencias
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.subject.classification
Medicina Básica
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.title
Raloxifene Protects Oxygen-glucose-deprived Astrocyte Cells Used To Mimic Hypoxic-ischemic Brain Injury
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2024-06-24T10:09:45Z
dc.identifier.eissn
2310-287X
dc.journal.pagination
1-15
dc.journal.pais
Suiza
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.journal.ciudad
Basel
dc.description.fil
Fil: Toro Urrego, Nicolas. Universidad Abierta Interamericana; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Luaces, Juan Pablo. Universidad Abierta Interamericana; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Bordet, Sofía. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires"; Argentina. Universidad Abierta Interamericana; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Otero-losada, Matilde Estela. Universidad Abierta Interamericana; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Capani, Francisco. Universidad Autónoma de Chile; Chile. Universidad Abierta Interamericana; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.journal.title
Preprints.org
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.preprints.org/manuscript/202405.1327/v1
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.20944/preprints202405.1327.v1
Archivos asociados