Artículo
Characterization of Binding Properties of Cr(Phen) 3 3+ and Ru(Phen) 3 2+ Complexes with Human Lactoferrin
Garcia, Pablo Facundo
; Saez Torillo, Santiago N.; Anzani, Angel Federico
; Arguello, Gerardo
; Burgos Paci, Maximiliano Alberto
![Icon](/themes/CONICETDigital/images/conicet.png)
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Fecha de publicación:
12/2022
Editorial:
Wiley Blackwell Publishing, Inc
Revista:
Photochemistry and Photobiology
ISSN:
0031-8655
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
This work presents research about [Cr(phen)3]3+ and [Ru(phen)3]2+ interaction with human lactoferrin (HLf), a key carrier protein of ferric cations. The photochemical and photophysical properties of [Cr(phen)3]3+ and [Ru(phen)3]2+ have been widely studied in the last decades due to their potential use as photosensitizers in photodynamic therapy (PDT). The behavior between the complexes and the protein was studied employing UV–visible absorption, fluorescence emission and circular dichroism spectroscopic techniques. It was found that both complexes bind to HLf with a large binding constant (Kb): 9.46 × 104 for the chromium complex and 4.16 × 104 for the ruthenium one at 299 K. Thermodynamic parameters were obtained from the Van´t Hoff equation. Analyses of entropy (ΔS), enthalpy (ΔH) and free energy changes (ΔG) indicate that these complexes bind to HLf because of entropy-driven processes and electrostatic interactions. According to circular dichroism experiments, no conformational changes have been observed in the secondary and tertiary structure of the protein in the presence of any of the studied complexes. These experimental results suggest that [Cr(phen)3]3+ and [Ru(phen)3]2+ bind to HLf, indicating that this protein could act as a carrier of these complexes in further applications.
Palabras clave:
Cr-Phen
,
TRANSITION METALS
,
PDT
,
SPECTROSCOPY
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(INFIQC)
Articulos de INST.DE INVESTIGACIONES EN FISICO- QUIMICA DE CORDOBA
Articulos de INST.DE INVESTIGACIONES EN FISICO- QUIMICA DE CORDOBA
Citación
Garcia, Pablo Facundo; Saez Torillo, Santiago N.; Anzani, Angel Federico; Arguello, Gerardo; Burgos Paci, Maximiliano Alberto; Characterization of Binding Properties of Cr(Phen) 3 3+ and Ru(Phen) 3 2+ Complexes with Human Lactoferrin; Wiley Blackwell Publishing, Inc; Photochemistry and Photobiology; 99; 5; 12-2022; 1225-1232
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