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dc.contributor.author
Santiago Valtierra, Florencia Ximena
dc.contributor.author
Urriola-Muñoz, Paulina
dc.contributor.author
Godoy-Sepúlveda, Rodrigo
dc.contributor.author
Moreno, Ricardo D.
dc.contributor.author
Reyes, Juan G
dc.contributor.author
Valles, Ana Sofia
dc.contributor.author
Oresti, Gerardo Martin
dc.date.available
2024-07-29T17:40:37Z
dc.date.issued
2024-06-13
dc.identifier.citation
Santiago Valtierra, Florencia Ximena; Urriola-Muñoz, Paulina; Godoy-Sepúlveda, Rodrigo; Moreno, Ricardo D.; Reyes, Juan G; et al.; Nonylphenol releases arachidonic acid in rat Sertoli cells via activation of PKA and PLA2; BioScientifica; Reproduction; 168; 1; 13-6-2024; 1-16
dc.identifier.issn
1470-1626
dc.identifier.uri
http://hdl.handle.net/11336/241155
dc.description.abstract
Nonylphenol (NP), an endocrine-disrupting chemical, is an environmental contaminant, and many notorious effects on male fertility have been reported in animal models and wild-type species. Here, we evaluated the effects of NP in follicle-stimulating hormone (FSH) signal transduction pathways and lipid metabolism using an in vitro model of rat Sertoli cell (SC) primary culture. Results show that an acute (1 h) SC exposure to NP (10 µM) increased the intra- and extra-cellular concentrations of free fatty acids (FFAs), mainly arachidonic acid (20:4n-6). Phosphatidylinositol seemed to be the major phospholipid source of this 20:4n-6 release by activation of the protein kinase A (PKA)/cytoplasmic phospholipase A2 (cPLA2) pathway. NP also increased diacylglycerols (DAG) levels and the expression (mRNA) of cyclooxygenase 2 (Cox2) and prostaglandin E2 (PGE2) levels. It is noteworthy that accumulation of lipid droplets took place after 24 h NP exposition, which was prevented by both a PKA inhibitor and a PLA2 inhibitor. Like FSH, NP triggers the release of 20:4n-6, which is a substrate for PGE2 synthesis via PKA/PLA2 activation. In addition, NP induces the formation of DAG, which could be required as a cofactor of the PKC-mediated activation of the COX2 inflammatory pathway. Our findings suggest that NP alters lipid homeostasis in SCs by inducing the activation of pro-inflammatory pathways that may trigger adverse effects in testis physiology over time. Concomitantly, the SC enhances the acylation of surplus FFAs (including 20:4n-6) in neutral lipids as a protective mechanism to shield itself from lipotoxicity and pro-inflammatory signals
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
BioScientifica
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
NONYLPHENOL
dc.subject
SERTOLI CELL
dc.subject
ARACHIDONIC ACID
dc.subject
PKA
dc.subject.classification
Bioquímica y Biología Molecular
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Nonylphenol releases arachidonic acid in rat Sertoli cells via activation of PKA and PLA2
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2024-07-10T14:07:26Z
dc.identifier.eissn
1741-7899
dc.journal.volume
168
dc.journal.number
1
dc.journal.pagination
1-16
dc.journal.pais
Reino Unido
dc.journal.ciudad
Btistol
dc.description.fil
Fil: Santiago Valtierra, Florencia Ximena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
dc.description.fil
Fil: Urriola-Muñoz, Paulina. Pontificia Universidad Católica de Chile; Chile
dc.description.fil
Fil: Godoy-Sepúlveda, Rodrigo. Pontificia Universidad Católica de Chile; Chile
dc.description.fil
Fil: Moreno, Ricardo D.. Pontificia Universidad Católica de Chile; Chile
dc.description.fil
Fil: Reyes, Juan G. Pontificia Universidad Católica de Valparaíso; Chile
dc.description.fil
Fil: Valles, Ana Sofia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
dc.description.fil
Fil: Oresti, Gerardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
dc.journal.title
Reproduction
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://rep.bioscientifica.com/view/journals/rep/168/1/REP-23-0292.xml
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1530/REP-23-0292
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