Artículo
EROD activity in the native fish Cnesterodon decemmaculatus as a biomarker for assessing aquatic pollution by AhR agonist chemicals within the Rio de la Plata Basin
Rabuffetti, Gabriela
; Brola, Jessica Nadia
; Perez, Daniela Edith
; Somoza, Gustavo Manuel
; Carriquiriborde, Pedro
Fecha de publicación:
07/2024
Editorial:
Elsevier Science
Revista:
Science of the Total Environment
ISSN:
0048-9697
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
The 7-ethoxyresorufin-O-deethylase (EROD) activity was first time characterized in the neotropical fish Cnesterodon decemmaculatus as a biomarker for assessing environmental health in aquatic ecosystems of the Rio de la Plata Basin impacted by organic pollutants agonist of the aryl-hydrocarbon receptor (AhR). Both laboratory and field studies were conducted. Laboratory experiments were run using β-naphthoflavone (BNF) as an AhR agonist model. A clear concentration-response relationship was found between 1 and 100 µg/L, with a NOEC and LOEC of 1 and 10 µg/L. A fast time-dependent response was observed with a significant induction after 24 h and a plateau from 24-48 h up to 264 h of exposure. Differences in basal activity were found between juveniles, females, and males, but induction levels were similar. Both basal activities and induction levels were distinct in the whole body, liver, gill, muscle, brain, and embryos. Fold-change inductions in the respective tissues were: 20, 114, 3, 5, 1, and 14. Maternal transfer and early cyp1a activation were unveiled by embryonic induction. Clear differences in EROD activity were foundamong juveniles collected in hydrocarbon-polluted streams, beside the La Plata Petrochemical hub, and a reference stream. Similar EROD activities were observed in laboratory and feral fish, usually with values below or above 1000 pmol/min x mg protein for unexposed or exposed organisms. The study contributes with original information about EROD activity in C. decemmaculatus that encourages the use of both the response as a robust biomarker of exposure and the species as a good sentinel organism to be included in surveillant programs for assessing aquatic pollution by AhR agonist chemicals within the Rio de la Plata Basin within the One Health paradigm.
Palabras clave:
ENVIRONMENTAL HEALTH
,
AQUATIC POLLUTION
,
CYROCHROME P450
,
BIOMARKERS
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos (CIM)
Articulos de CENTRO DE INVESTIGACIONES DEL MEDIO AMBIENTE
Articulos de CENTRO DE INVESTIGACIONES DEL MEDIO AMBIENTE
Articulos(CCT - LA PLATA)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - LA PLATA
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - LA PLATA
Citación
Rabuffetti, Gabriela; Brola, Jessica Nadia; Perez, Daniela Edith; Somoza, Gustavo Manuel; Carriquiriborde, Pedro; EROD activity in the native fish Cnesterodon decemmaculatus as a biomarker for assessing aquatic pollution by AhR agonist chemicals within the Rio de la Plata Basin; Elsevier Science; Science of the Total Environment; 947; 7-2024; 1-8
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