Mineral fertilization impacts microbial activity and endophytic fungi but not microbial biomass in semiarid grasslands

Abstract

Applications of mineral fertilizer to grasslands have become more frequent in recent decades to increase forage production. However, the impacts of mineral fertilizer on the soil microbiome is poorly understood in cold semiarid grassland ecosystems of Southern Patagonia, Argentina. Therefore, our objective was to analyze experimentally the influence of mineral nutrient fertilization (N, P, K, and NPK in combination) on soil microbial community attributes, such as microbial biomass carbon (MBC) and nitrogen (MBN), soil basal respiration (SBR), microbial metabolic coefficients, the colonization of endophytic fungi such as arbuscular mycorrhizal (AM) fungi, and dark septate endophytes (DSE), and aboveground plant productivity. Mineral fertilization with macronutrients (N, P, K, and NPK) decreased the SBR, qCO2, AM fungi and DSE fungi, but did not generate changes in MBC and MBN. The magnitude of these responses depends on years after fertilization. We found that soil microbiome was strongly dependent on a range of biotic and abiotic actors, such as growing season precipitation, aboveground plant biomass the relationship between the microbial biomass and microbial respiration, and between endophytic fungi and plants. This work improved our understanding of the soil microorganisms? response to mineral fertilizer application and provides new insights into soil nutrient dynamics and ecosystem functioning.