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dc.contributor.author
del Giudice, Mariela Giselda
dc.contributor.author
Ugalde, Juan Esteban
dc.contributor.author
Czibener, Cecilia
dc.date.available
2017-09-12T18:27:02Z
dc.date.issued
2013-03
dc.identifier.citation
del Giudice, Mariela Giselda; Ugalde, Juan Esteban; Czibener, Cecilia; A lysozyme-like protein in Brucella abortus is involved in the early stages of intracellular replication; American Society for Microbiology; Infection and Immunity; 81; 3; 3-2013; 956-964
dc.identifier.issn
0019-9567
dc.identifier.uri
http://hdl.handle.net/11336/24060
dc.description.abstract
Secretion of proteins in Gram-negative bacteria is a high-energy consuming process that requires the translocation across two membranes and a periplasmic space composed of a mesh like layer, the peptidoglycan. To achieve this, bacteria have evolved complex secretion systems that cross these barriers and, in many cases, there are specific peptidoglycanases that degrade the peptidoglycan to allow the proper assembly of the secretion machinery. We describe here the identification and characterization of a muramidase in Brucella abortus that participates in the intracellular multiplication in professional and non-professional phagocytes. We demonstrated that this protein has peptidoglycanase activity, that a strain with a clean deletion of the gene displayed a defect in the early stages of the intracellular multiplication curve and that this is dependent on the lytic activity. While neither the attachment nor the invasion of the strain was affected we demonstrated that it had a defect in excluding the lysosomal marker LAMP-1 but not in acquiring the reticulum endoplasmic marker calnexin, indicating that the gene participates in the early stages of the intracellular trafficking but not in the establishment of the replicative niche. Analysis of the assembly status and functionality of the VirB secretion apparatus indicated that the mutant has affected the proper function of this central virulence factor.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
American Society for Microbiology
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Brucella Abortus
dc.subject
Lysozyme
dc.subject.classification
Bioquímica y Biología Molecular
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
A lysozyme-like protein in Brucella abortus is involved in the early stages of intracellular replication
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-09-06T17:19:13Z
dc.journal.volume
81
dc.journal.number
3
dc.journal.pagination
956-964
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Washington
dc.description.fil
Fil: del Giudice, Mariela Giselda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
dc.description.fil
Fil: Ugalde, Juan Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
dc.description.fil
Fil: Czibener, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
dc.journal.title
Infection and Immunity
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1128/IAI.01158-12
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://iai.asm.org/content/81/3/956
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