Artículo
Origami Paper-Based Electrochemical Immunosensor with Carbon Nanohorns-Decorated Nanoporous Gold for Zearalenone Detection
Laza Correa, Anabel
; Pereira, Sirley Vanesa
; Messina, Germán Alejandro
; Fernández Baldo, Martín Alejandro
; Raba, Julio
; Regiart, Daniel Matias Gaston
; Bertolino, Franco Adrián
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Fecha de publicación:
01/2024
Editorial:
Multidisciplinary Digital Publishing Institute
Revista:
Chemosensors
ISSN:
2227-9040
e-ISSN:
2227-9040
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Nowadays, mycotoxin contamination in cereals and wastewater exposes a safety hazard to consumer health. This work describes the design of a simple, low-cost, and sensitive origami microfluidic paper-based device using electrochemical detection for zearalenone determination. The microfluidic immunosensor was designed on a paper platform by a wax printing process. The graphitized carbon working electrode modified with carbon nanohorns-decorated nanoporous gold showed a higher surface area, sensitivity, and adequate analytical performance. Electrodes were characterized by scanning electron microscopy, energy-dispersive spectroscopy, and cyclic voltammetry. The determination of zearalenone was carried out through a competitive immunoassay using specific antibodies immobilized by a covalent bond on the electrode surface. In the presence of HRP-labeled enzyme conjugate, substrate, and catechol, zearalenone was detected employing the developed immunosensor by applying −0.1 V to the working electrode vs silver as a pseudo-reference electrode. A calibration curve with a linear range between 10 and 1000 µg Kg−1 (R2 = 0.998) was obtained, and the limit of detection and quantification for the electrochemical immunosensor were 4.40 and 14.90 µg Kg−1 , respectively. The coefficient of variation for intra- and inter-day assays was less than 5%. The selectivity and specificity of the sensor were evaluated, comparing the response against zearalenone metabolites and other mycotoxins that could affect the corn samples. Therefore, origami is a promising approach for paper-based electrochemical microfluidic sensors coupled to smartphones as a rapid and portable tool for in situ mycotoxins detection in real samples.
Palabras clave:
PAPER-BASED
,
ELECTROCHEMICAL
,
INMUNOSENSOR
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(INQUISAL)
Articulos de INST. DE QUIMICA DE SAN LUIS
Articulos de INST. DE QUIMICA DE SAN LUIS
Citación
Laza Correa, Anabel; Pereira, Sirley Vanesa; Messina, Germán Alejandro; Fernández Baldo, Martín Alejandro; Raba, Julio; et al.; Origami Paper-Based Electrochemical Immunosensor with Carbon Nanohorns-Decorated Nanoporous Gold for Zearalenone Detection; Multidisciplinary Digital Publishing Institute; Chemosensors; 12; 1; 1-2024; 1-12
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