Artículo
Integrated hepatitis e virus monitoring in central Argentina: a six-year analysis of clinical surveillance and wastewater-based epidemiology
Fantilli, Anabella Clara
; Masachessi, Gisela
; Di Cola Bucciarelli, Guadalupe
; Castro, Gonzalo Manuel
; Sicilia, Paola; Marinzalda, María de Los Angeles
; Cachi, Ariana Mariela; Moreno, Claudia; Borda, Mariel Alejandra; Ibarra, Gustavo; Rojas, Ricardo Manuel; Parreño, Gladys Viviana
; Barbas, Maria Gabriela; Nates, Silvia Viviana; Pisano, María Belén
; Ré, Viviana Elizabeth
Fecha de publicación:
09/2024
Editorial:
Pergamon-Elsevier Science Ltd
Revista:
Water Research
ISSN:
0043-1354
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Wastewater-based epidemiology (WBE) has gained prominence worldwide as a powerful tool in public health. This study aimed to monitor the circulation of Hepatitis E Virus (HEV) from wastewater samples collected during a six-year period and compare these results with clinical surveillance in the central region of Argentina. From 2017 to 2022, 1008 raw wastewater samples were analyzed, including four wastewater treatment plants from four cities (n=319), and 7 local neighborhood collector sewers in Córdoba city (n=689). Serum and/or stool samples from patients suspected of HEV infection were also analyzed (n=48). HEV molecular detection and viral load quantification were performed by real time RT-qPCR, and genetic characterization by two RT-Nested PCRs (targeting partial ORF-1 and ORF-2 genomic regions), sequencing and phylogenetic analysis. Fifty-three (5.3%) wastewater samples were RNA-HEV positive by real time RT-qPCR, with variations according to the location and year (0.0% - 21.6%). Out of these, ORF-2 genomic region was amplified in 20 samples (37.7%) and ORF-1 partial region in 12 (22.6%), and eighteen sequences were obtained. Throughout the study period, two (4.2%) HEV confirmed infections were reported, and one sequence was obtained. Phylogenetic analyses for both genomic regions showed that all the isolates were genotype HEV-3 clade abchijklm. Our study detected HEV in wastewater over a six-year period, despite a low number of clinical cases, emphasizing WBE as a valuable tool that complements clinical surveillance, by detecting pathogens´ presence; identifying their transmission, circulation dynamics and excretion hotspots; and revealing changes in their genomic diversity.
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Licencia
Identificadores
Colecciones
Articulos (IVIT)
Articulos de INSTITUTO DE VIROLOGIA E INNOVACIONES TECNOLOGICAS
Articulos de INSTITUTO DE VIROLOGIA E INNOVACIONES TECNOLOGICAS
Articulos(CCT - CORDOBA)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - CORDOBA
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - CORDOBA
Citación
Fantilli, Anabella Clara; Masachessi, Gisela; Di Cola Bucciarelli, Guadalupe; Castro, Gonzalo Manuel; Sicilia, Paola; et al.; Integrated hepatitis e virus monitoring in central Argentina: a six-year analysis of clinical surveillance and wastewater-based epidemiology; Pergamon-Elsevier Science Ltd; Water Research; 261; 9-2024; 1-10
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