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dc.contributor.author
Alvau, Antonio
dc.contributor.author
Battistone, Maria Agustina
dc.contributor.author
Gervasi, Maria Gracia
dc.contributor.author
Navarrete, Felipe A.
dc.contributor.author
Xu, Xinran
dc.contributor.author
Sánchez Cárdenas, Claudia
dc.contributor.author
De la Vega Beltran, José Luis
dc.contributor.author
Da Ros, Vanina Gabriela
dc.contributor.author
Greer, Peter
dc.contributor.author
Darszon, Alberto
dc.contributor.author
Krapf, Diego
dc.contributor.author
Salicioni, Ana María
dc.contributor.author
Cuasnicu, Patricia Sara
dc.contributor.author
Visconti, Pablo E.
dc.date.available
2017-09-12T17:54:32Z
dc.date.issued
2016-07
dc.identifier.citation
Alvau, Antonio; Battistone, Maria Agustina; Gervasi, Maria Gracia; Navarrete, Felipe A.; Xu, Xinran; et al.; The tyrosine kinase FER is responsible for the capacitation-associated increase in tyrosine phosphorylation in murine sperm; Company of Biologists; Development; 143; 13; 7-2016; 2325-2333
dc.identifier.issn
0950-1991
dc.identifier.uri
http://hdl.handle.net/11336/24029
dc.description.abstract
Sperm capacitation is required for fertilization. At the molecular level, this process is associated with fast activation of protein kinase A. Downstream of this event, capacitating conditions lead to an increase in tyrosine phosphorylation. The identity of the tyrosine kinase(s) mediating this process has not been conclusively demonstrated. Recent experiments using stallion and human sperm have suggested a role for PYK2 based on the use of small molecule inhibitors directed against this kinase. However, crucially, loss-of-function experiments have not been reported. Here, we used both pharmacological inhibitors and genetically modified mice models to investigate the identity of the tyrosine kinase(s) mediating the increase in tyrosine phosphorylation in mouse sperm. Similar to stallion and human, PF431396 blocks the capacitation-associated increase in tyrosine phosphorylation. Yet, sperm from Pyk2(-/-) mice displayed a normal increase in tyrosine phosphorylation, implying that PYK2 is not responsible for this phosphorylation process. Here, we show that PF431396 can also inhibit FER, a tyrosine kinase known to be present in sperm. Sperm from mice targeted with a kinase-inactivating mutation in Fer failed to undergo capacitation-associated increases in tyrosine phosphorylation. Although these mice are fertile, their sperm displayed a reduced ability to fertilize metaphase II-arrested eggs in vitro.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Company of Biologists
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Fer
dc.subject
Sperm Capacitation
dc.subject
Tyrosine Phosphorylation
dc.subject.classification
Bioquímica y Biología Molecular
dc.subject.classification
Medicina Básica
dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
The tyrosine kinase FER is responsible for the capacitation-associated increase in tyrosine phosphorylation in murine sperm
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-08-31T20:30:37Z
dc.identifier.eissn
1477-9129
dc.journal.volume
143
dc.journal.number
13
dc.journal.pagination
2325-2333
dc.journal.pais
Reino Unido
dc.journal.ciudad
Cambridge
dc.description.fil
Fil: Alvau, Antonio. University of Massachussets; Estados Unidos
dc.description.fil
Fil: Battistone, Maria Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
dc.description.fil
Fil: Gervasi, Maria Gracia. University of Massachussets; Estados Unidos
dc.description.fil
Fil: Navarrete, Felipe A.. University of Massachussets; Estados Unidos
dc.description.fil
Fil: Xu, Xinran. State University of Colorado - Fort Collins; Estados Unidos
dc.description.fil
Fil: Sánchez Cárdenas, Claudia. Universidad Nacional Autónoma de México. Instituto de Biotecnología; México
dc.description.fil
Fil: De la Vega Beltran, José Luis. Universidad Nacional Autónoma de México. Instituto de Biotecnología; México
dc.description.fil
Fil: Da Ros, Vanina Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
dc.description.fil
Fil: Greer, Peter. Queens University; Canadá
dc.description.fil
Fil: Darszon, Alberto. Universidad Nacional Autónoma de México. Instituto de Biotecnología; México
dc.description.fil
Fil: Krapf, Diego. State University of Colorado - Fort Collins; Estados Unidos
dc.description.fil
Fil: Salicioni, Ana María. University of Massachussets; Estados Unidos
dc.description.fil
Fil: Cuasnicu, Patricia Sara. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
dc.description.fil
Fil: Visconti, Pablo E.. University of Massachussets; Estados Unidos
dc.journal.title
Development
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://dev.biologists.org/content/143/13/2325
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1242/dev.136499
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/pmid/27226326


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