Repositorio Institucional
Repositorio Institucional
CONICET Digital
  • Inicio
  • EXPLORAR
    • AUTORES
    • DISCIPLINAS
    • COMUNIDADES
  • Estadísticas
  • Novedades
    • Noticias
    • Boletines
  • Ayuda
    • General
    • Datos de investigación
  • Acerca de
    • CONICET Digital
    • Equipo
    • Red Federal
  • Contacto
JavaScript is disabled for your browser. Some features of this site may not work without it.
  • INFORMACIÓN GENERAL
  • RESUMEN
  • ESTADISTICAS
 
Artículo

Improvement of chicken primordial germ cell maintenance in vitro by blockade of the Aryl Hydrocarbon Receptor endogenous activity

Pérez Sáez, Juan Manuel; Bussmann, Leonardo EdmundoIcon ; Barañao, Jose Lino SalvadorIcon ; Bussmann, Ursula AgnesIcon
Fecha de publicación: 06/2016
Editorial: Mary Ann Liebert Inc
Revista: Cellular Reprogramming
ISSN: 2152-4971
e-ISSN: 2152-4998
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Biología Reproductiva; Bioprocesamiento Tecnológico, Biocatálisis, Fermentación

Resumen

Primordial germ cells (PGCs) are the undifferentiated progenitors of gametes. Germline competent PGCs can be developed as a cell-based system for genetic modification in chickens, which provides a valuable tool for transgenic technology with both research and industrial applications. This implies manipulation of PGCs, which encouraged in recent years a lot of research focused on the study of PGCs and the way of improving their culture. The aryl hydrocarbon receptor (AHR) is a ligand-activated transcription factor that besides mediating toxic responses to environmental contaminants plays pivotal physiological roles in various biological processes. Since a novel compound that acts as an antagonist of this receptor has been reported to promote expansion of hematopoietic stem cells, we conducted the present study with the aim of determining if addition of an established AHR antagonist to the standard culture medium used nowadays for in vitro chicken PGCs culture improves ex vivo expansion. We have found that addition of α-naphthoflavone in culture medium promotes the amplification of undifferentiated cells and that this effect is exerted by blockade of AHR action. Our results constitute the first report of the successful use of a readily available AHR antagonist to improve avian PGCs expansion and they further extend the knowledge of the effects of AHR modulation in undifferentiated cells.
Palabras clave: Aryl Hydrocarbon Receptor , Alpha-Naphthoflavone , Chicken , Primordial Germ Cell
Ver el registro completo
 
Archivos asociados
Thumbnail
 
Tamaño: 538.1Kb
Formato: PDF
.
Descargar
Licencia
info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/23989
URL: http://online.liebertpub.com/doi/full/10.1089/cell.2016.0015
DOI: http://dx.doi.org/10.1089/cell.2016.0015
Colecciones
Articulos(IBYME)
Articulos de INST.DE BIOLOGIA Y MEDICINA EXPERIMENTAL (I)
Citación
Pérez Sáez, Juan Manuel; Bussmann, Leonardo Edmundo; Barañao, Jose Lino Salvador; Bussmann, Ursula Agnes; Improvement of chicken primordial germ cell maintenance in vitro by blockade of the Aryl Hydrocarbon Receptor endogenous activity; Mary Ann Liebert Inc; Cellular Reprogramming; 18; 3; 6-2016; 154-161
Compartir
Altmétricas
 

Enviar por e-mail
Separar cada destinatario (hasta 5) con punto y coma.
  • Facebook
  • X Conicet Digital
  • Instagram
  • YouTube
  • Sound Cloud
  • LinkedIn

Los contenidos del CONICET están licenciados bajo Creative Commons Reconocimiento 2.5 Argentina License

https://www.conicet.gov.ar/ - CONICET

Inicio

Explorar

  • Autores
  • Disciplinas
  • Comunidades

Estadísticas

Novedades

  • Noticias
  • Boletines

Ayuda

Acerca de

  • CONICET Digital
  • Equipo
  • Red Federal

Contacto

Godoy Cruz 2290 (C1425FQB) CABA – República Argentina – Tel: +5411 4899-5400 repositorio@conicet.gov.ar
TÉRMINOS Y CONDICIONES