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dc.contributor.author Pinedo, Marcela Lilian
dc.contributor.author Lechner, L.
dc.contributor.author Creus, Cecilia Mónica
dc.contributor.author Simontacchi, Marcela Silvia
dc.contributor.author Aguirrezábal, Luis Adolfo Nazareno
dc.date.available 2017-09-12T14:06:28Z
dc.date.issued 2012-10-08
dc.identifier.citation Pinedo, Marcela Lilian; Lechner, L.; Creus, Cecilia Mónica; Simontacchi, Marcela Silvia; Aguirrezábal, Luis Adolfo Nazareno; Endogenous ascorbate restrains apoplastic peroxidase activity during sunflower leaf development; Wiley Blackwell Publishing, Inc; Plant Biology; 15; 1; 8-10-2012; 60-66
dc.identifier.issn 1435-8603
dc.identifier.uri http://hdl.handle.net/11336/23976
dc.description.abstract Several apoplastic enzymes have been implicated in the control of elongation growth of plant cells. Among them, peroxidases contribute to both loosening and stiffening of the cell wall. They appear to be regulated by various mechanisms, including the action of extracellular inhibitors. To obtain evidence of the role of the enzyme–inhibitor interaction during leaf development, the intercellular washing fluids from Helianthus annuus leaves of different ages were isolated using standard methods of vacuum infiltration and centrifugation. Peroxidase activities, assessed using tetramethylbenzidine as substrate, increased during leaf development, reaching a maximum value after the leaves were fully expanded. An inhibitor, chemically characterised as ascorbate, co-localised with the enzyme in the apoplast. Moreover, there was a strong negative correlation between the action of peroxidase and the micromolar concentration of ascorbate in the apoplastic fluid. The results show that in growing leaves, the in planta ascorbate concentration is able to restrain peroxidase enzyme activity. Then, at the time of growth cessation, the loss of extracellular ascorbate relieves the inhibition on this enzyme that contributes to wall fixation.
dc.format application/pdf
dc.language.iso eng
dc.publisher Wiley Blackwell Publishing, Inc
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject Ascorbate
dc.subject cell wall
dc.subject extracellular
dc.subject inhibitor
dc.subject leaf growth
dc.subject peroxidase
dc.subject sunflower
dc.subject.classification Bioquímica y Biología Molecular
dc.subject.classification Ciencias Biológicas
dc.subject.classification CIENCIAS NATURALES Y EXACTAS
dc.title Endogenous ascorbate restrains apoplastic peroxidase activity during sunflower leaf development
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2017-08-29T19:57:42Z
dc.journal.volume 15
dc.journal.number 1
dc.journal.pagination 60-66
dc.journal.pais Reino Unido
dc.journal.ciudad Londres
dc.description.fil Fil: Pinedo, Marcela Lilian. Universidad Nacional de Mar del Plata; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil Fil: Lechner, L.. Universidad Nacional de Mar del Plata; Argentina. Instituto Nacional de Tecnología Agropecuaria; Argentina
dc.description.fil Fil: Creus, Cecilia Mónica. Instituto Nacional de Tecnología Agropecuaria; Argentina. Universidad Nacional de Mar del Plata; Argentina
dc.description.fil Fil: Simontacchi, Marcela Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; Argentina
dc.description.fil Fil: Aguirrezábal, Luis Adolfo Nazareno. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; Argentina
dc.journal.title Plant Biology
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1111/j.1438-8677.2012.00620.x
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/j.1438-8677.2012.00620.x/abstract?


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info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)