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dc.contributor.author
Gangoiti, Maria Virginia  
dc.contributor.author
Arnol, Verònica  
dc.contributor.author
Cortizo, Ana María  
dc.contributor.author
McCarthy, Antonio Desmond  
dc.date.available
2017-09-11T21:19:13Z  
dc.date.issued
2013-07  
dc.identifier.citation
Gangoiti, Maria Virginia; Arnol, Verònica; Cortizo, Ana María; McCarthy, Antonio Desmond; Advanced Glycation Endproducts and Alendronate Differentially Inhibit early and Late Osteoclastogenesis In vitro; OMICS; Journal of diabetes and metabolism; 4; 6; 7-2013; 1-7  
dc.identifier.issn
2155-6156  
dc.identifier.uri
http://hdl.handle.net/11336/23956  
dc.description.abstract
Advanced Glycation Endproducts (AGEs) are greatly elevated in bone extracellular matrix of patients with Diabetes mellitus, and this has been associated with the increased incidence of fractures observed in these patients. AGEs affect the homeostasis of bone cells such as osteoblasts and osteoclasts. Bisphosphonates are first-line anti-osteoporotic drugs that principally exert their effects by inhibiting osteoclastic activity. However, the effect of bisphosphonate treatment on bone quality in patients with Diabetes is uncertain. In the present work we have evaluated the action of AGEs (50-200 μg/ml), with or without Alendronate (10-8-10-4M), on osteoclastogenesis induced by co-cultures of Raw 264.7 macrophages and UMR106 osteoblasts. We determined the effects of different culture conditions on osteoclastic recruitment, tartrate-resistant acid phosphatase (TRAP) activity and expression of RAGE (receptor for AGEs); and on the osteoblastic expression of RANK ligand (RANKL). AGEs and Alendronate inhibited the recruitment and TRAP activity of osteoclasts, with an additive effect of both agents at high concentrations of Alendronate. While AGEs prevented the early and late stages of osteoclastogenesis, Alendronate (alone or in co-incubation with AGEs) only inhibited its later stages. In addition, both AGEs and Alendronate increased the osteoclastic expression of RAGE and decreased the osteoblastic expression of RANKL, correlating closely with their inhibition of osteoclastogenesis. If these in vitro results can be extrapolated to a clinical setting, they may be indicating a potentiation of the anti-resorptive effects of Alendronate in the context of bone extracellular matrix with excess accumulation of AGEs, as might occur in a patient with Diabetes mellitus.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
OMICS  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
Advanced Glycation Endproducts  
dc.subject
Osteoclasts  
dc.subject
Alendronate  
dc.subject
Rage  
dc.subject
Rankl  
dc.subject.classification
Bioquímica y Biología Molecular  
dc.subject.classification
Medicina Básica  
dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD  
dc.title
Advanced Glycation Endproducts and Alendronate Differentially Inhibit early and Late Osteoclastogenesis In vitro  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2017-08-23T20:36:36Z  
dc.journal.volume
4  
dc.journal.number
6  
dc.journal.pagination
1-7  
dc.journal.pais
Estados Unidos  
dc.journal.ciudad
Sunnyvale  
dc.description.fil
Fil: Gangoiti, Maria Virginia. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación en Osteospatías y Metabolismo Mineral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina  
dc.description.fil
Fil: Arnol, Verònica. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación en Osteospatías y Metabolismo Mineral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina  
dc.description.fil
Fil: Cortizo, Ana María. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación en Osteospatías y Metabolismo Mineral; Argentina  
dc.description.fil
Fil: McCarthy, Antonio Desmond. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación en Osteospatías y Metabolismo Mineral; Argentina  
dc.journal.title
Journal of diabetes and metabolism  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.4172/2155-6156.1000274  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.omicsonline.org/2155-6156/2155-6156-4-274.php?aid=16053