Artículo
Novel tnpR-based transposable promoter traps suitable for RIVET studies in different gram-negative bacteria
Lozano, Mauricio Javier
; Salas, María Eugenia
; Giusti, María de Los Ángeles
; Martini, María Carla
; López, José Luis
; Salto, Ileana Paula
; del Papa, Maria Florencia
; Pistorio, Mariano
; Lagares, Antonio
Fecha de publicación:
02/2013
Editorial:
Elsevier Science
Revista:
Journal Of Microbiological Methods
ISSN:
0167-7012
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Resumen
The preparation of plasmid-borne RIVET libraries can be troublesome when high genomic coverages are needed. We present here the construction and functional validation of a new set of miniTn5 promoter traps to generate tnpR-based RIVET libraries. The ability to generate tnpR transcriptional fusions by transposition will significantly facilitate the setup of RIVET studies in those bacteria where Tn5 transposition is operative.
Palabras clave:
Minitn5
,
Rivet
,
Tnpr
,
Promoter Probe
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Articulos(IBBM)
Articulos de INST.DE BIOTECNOLOGIA Y BIOLOGIA MOLECULAR
Articulos de INST.DE BIOTECNOLOGIA Y BIOLOGIA MOLECULAR
Citación
Lozano, Mauricio Javier; Salas, María Eugenia; Giusti, María de Los Ángeles; Martini, María Carla; López, José Luis; et al.; Novel tnpR-based transposable promoter traps suitable for RIVET studies in different gram-negative bacteria; Elsevier Science; Journal Of Microbiological Methods; 93; 1; 2-2013; 9-11
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