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dc.contributor.author
Arshad, Usman
dc.contributor.author
Zenobi, Marcos G.
dc.contributor.author
Tribulo, Paula
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dc.contributor.author
Staples, Charles R.
dc.contributor.author
Santos, José E. P.
dc.date.available
2024-07-01T11:49:25Z
dc.date.issued
2023-10
dc.identifier.citation
Arshad, Usman; Zenobi, Marcos G.; Tribulo, Paula; Staples, Charles R.; Santos, José E. P.; Dose-dependent effects of rumen-protected choline on hepatic metabolism during induction of fatty liver in dry pregnant dairy cows; Public Library of Science; Plos One; 18; 10; 10-2023; 1-20
dc.identifier.issn
1932-6203
dc.identifier.uri
http://hdl.handle.net/11336/238672
dc.description.abstract
Objectives were to determine the effects of supplementing increasing amounts of choline ion on hepatic composition and mRNA abundance in pregnant dry cows subjected to a fatty liver induction protocol. Holstein cows (35 primiparous and 41 multiparous) at mean (± standard deviation) of 211 ± 9.9 days of gestation were blocked by body condition (3.59 ± 0.33) andassigned to receive 0, 6.45, 12.90, 19.35, and 25.80 g/day of choline ion as rumen-protected choline (RPC) as a top-dress for 14 days. Cows were fed for ad libitum intake on days 1 to 5andrestricted to 30% of the required net energy for lactation from days 6 to 14 of the experiment. Hepatic tissue was sampled on days 5 and 14 and analyzed for concentrations of triacylglycerol and glycogen, and mRNA abundance was investigated. Orthogonal contrasts evaluated the effects of supplementing RPC (0 g/day vs. rest), and the linear, quadratic, and cubic effects of increasing intake of choline ion from 6.45 to 25.80 g/day. Results are depicted in sequence of treatments from 0 to 25.8. During feed restriction, RPC reduced the concentration of hepatic triacylglycerol by 28.5% and increased that of glycogen by 26.1%, and the effect of increasing RPC intake on triacylglycerol was linear (6.67 vs. 5.45 vs. 4.68 vs. 5.13 vs. 3.81 ± 0.92% wet-basis). Feeding RPC during feed restriction increased abundance of transcripts involved in choline metabolism (CHKA, PLD1), synthesis of apolipoprotein-B100 (APOB100), and antioxidant activity (GPX3), and decreased the abundance of transcripts involved in hepatic lipogenesis (DGAT2, SREBF1) and acute phase response (SAA3). Most effects were linear with amount of choline fed. Changes in hepatic mRNA abundance followed a pattern of reduced lipogenesis and enhanced lipids export, which help explain the reduced hepatic triacylglycerol content in cows fed RPC. Choline exerts lipotropic effects in dairy cows by altering transcript pathways linked to hepatic lipids metabolism.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Public Library of Science
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
VACA LECHERA
dc.subject
HIGADO GRASO
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ARN MENSAJERO
dc.subject
COLINA PROTEGIDA DE DEGRADACION RUMINAL
dc.subject.classification
Producción Animal y Lechería
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dc.subject.classification
Producción Animal y Lechería
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dc.subject.classification
CIENCIAS AGRÍCOLAS
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dc.title
Dose-dependent effects of rumen-protected choline on hepatic metabolism during induction of fatty liver in dry pregnant dairy cows
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2024-07-01T10:21:12Z
dc.journal.volume
18
dc.journal.number
10
dc.journal.pagination
1-20
dc.journal.pais
Estados Unidos
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dc.journal.ciudad
San Francisco
dc.description.fil
Fil: Arshad, Usman. University of Florida; Estados Unidos
dc.description.fil
Fil: Zenobi, Marcos G.. Universidad Nacional de Córdoba; Argentina
dc.description.fil
Fil: Tribulo, Paula. Universidad Nacional de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
dc.description.fil
Fil: Staples, Charles R.. University of Florida; Estados Unidos
dc.description.fil
Fil: Santos, José E. P.. University of Florida; Estados Unidos
dc.journal.title
Plos One
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1371/journal.pone.0290562
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