Artículo
Screening of antimicrobial activity of Ilex paraguariensis St. Hil. leaf extracts against carbapenemase-producing bacteria
Onetto, Andrea Liliana
; Novosak, Marina Gisel
; Winnik, Daniana Lilian; Cortese, Iliana Julieta
; Stockmanns, Patricia Elizabeth
; Oviedo, Patricia Noemí; Laczeski, Margarita Ester
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Fecha de publicación:
06/2024
Editorial:
Academia Brasileira de Ciencias
Revista:
Anais da Academia Brasileira de Ciencias
ISSN:
0001-3765
e-ISSN:
1678-2690
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Ilex paraguariensis is a south American species of agronomic interest with studies supporting its medicinal properties. As the investigation of active ingredients with antimicrobial effect from medicinal plants is a suitable approach to the current antibacterial resistance problem, the aim of the present study was to determine the antibacterial activity of yerba mate ethanolic extracts against carbapenemase-producing gram-negative bacteria (reference strains and clinical isolates). Extracts showed antibacterial activity against Klebsiella pneumoniae ATCC® BAA-2342™ (KPC producing), Providencia rettgeri (NDM producing), Pseudomonas aeruginosa (MBL producing) and P. aeruginosa (VIM producing) at the concentrations tested. The Minimal-Inhibitory-Concentration and Minimal-Bactericidal-Concentration values ranged between 1 and 32 mg.ml-1 for the reference strains, and between 0.125 and 1 mg.ml-1 for the clinical isolates. The MBC/MIC index characterized the extracts as bactericidal. The combinations of commercial antibiotics and extracts showed a synergistic action on the reference strains studied. The lethal concentration 50 obtained using the Artemia salina toxicity assay were higher than 1 mg.ml-1 for all the extracts, indicating a low toxicity. The in vitro activity and low toxicity suggest that ethanolic I. paraguariensis leaf extracts constitute an outstanding source for new antibacterial compounds, and further studies should be carried out to understand their mechanism of action.
Palabras clave:
ANTIBACTERIAL ACTIVITY
,
ILEX PARAGUARIENSIS
,
MIC
,
MBC
,
PLANT EXTRACTS
,
SYNERGISM
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(CCT - NORDESTE)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - NORDESTE
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - NORDESTE
Citación
Onetto, Andrea Liliana; Novosak, Marina Gisel; Winnik, Daniana Lilian; Cortese, Iliana Julieta; Stockmanns, Patricia Elizabeth; et al.; Screening of antimicrobial activity of Ilex paraguariensis St. Hil. leaf extracts against carbapenemase-producing bacteria; Academia Brasileira de Ciencias; Anais da Academia Brasileira de Ciencias; 96; 3; 6-2024; 1-14
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