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dc.contributor.author
Jerez, Horacio Emanuel
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dc.contributor.author
Simioni, Yamila Roxana
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dc.contributor.author
Ghosal, Kajal
dc.contributor.author
Morilla, María José
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dc.contributor.author
Romero, Eder Lilia
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dc.date.available
2024-06-28T10:51:47Z
dc.date.issued
2024-05
dc.identifier.citation
Jerez, Horacio Emanuel; Simioni, Yamila Roxana; Ghosal, Kajal; Morilla, María José; Romero, Eder Lilia; Cholesterol nanoarchaeosomes for alendronate targeted delivery as an anti-endothelial dysfunction agent; Beilstein-Institut; Beilstein Journal of Nanotechnology; 15; 5-2024; 517-534
dc.identifier.issn
2190-4286
dc.identifier.uri
http://hdl.handle.net/11336/238557
dc.description.abstract
Sodium alendronate (ALN) is a very hydrosoluble and poorly permeable molecule used as an antiresorptive agent and with vascularanticalcifying capacity. Loaded into targeted nanovesicles, its anti-inflammatory activity may be amplified towards extra-osseousand noncalcified target cells, such as severely irritated vascular endothelium. Here cytotoxicity, mitochondrial membrane potential,ATP content, and membrane fluidity of human endothelial venous cells (HUVECs) were determined after endocytosis of ALNloadednanoarchaeosomes (nanoARC-Chol(ALN), made of polar lipids from Halorubrum tebenquichense: cholesterol 7:3 w/w,166 ± 5 nm, 0.16 ± 0.02 PDI, −40.8 ± 5.4 mV potential, 84.7 ± 21 μg/mg ALN/total lipids, TL). The effect of nanoARCChol(ALN) was further assessed on severely inflamed HUVECs. To that aim, HUVECs were grown on a porous barrier on top of abasal compartment seeded either with macrophages or human foam cells. One lighter and one more pronounced inflammatorycontext was modelled by adding lipopolysaccharide (LPS) to the apical or the apical and basal compartments. The endocytosis ofnanoARC-Chol(ALN), was observed to partly reduce the endothelial-mesenchymal transition of HUVECs. Besides, while10 mg/mL dexamethasone, 7.6 mM free ALN and ALN-loaded liposomes failed, 50 μg/mL TL + 2.5 μg/mL ALN (i.e., nanoARCChol(ALN)) reduced the IL-6 and IL-8 levels by, respectively, 75% and 65% in the mild and by, respectively, 60% and 40% in thepronounced inflammation model. This is the first report showing that the endocytosis of nanoARC-Chol(ALN) by HUVECs magnifiesthe anti-inflammatory activity of ALN even under conditions of intense irritation, not only surpassing that of free ALN but alsothat of dexamethasone.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Beilstein-Institut
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/
dc.subject
alendronate
dc.subject
archaeosomes
dc.subject
endothelial
dc.subject
inflammation
dc.subject.classification
Bioproductos, Biomateriales, Bioplásticos, Biocombustibles, Bioderivados, etc.
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dc.subject.classification
Biotecnología Industrial
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dc.subject.classification
INGENIERÍAS Y TECNOLOGÍAS
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dc.title
Cholesterol nanoarchaeosomes for alendronate targeted delivery as an anti-endothelial dysfunction agent
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2024-06-24T10:06:46Z
dc.journal.volume
15
dc.journal.pagination
517-534
dc.journal.pais
Alemania
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dc.description.fil
Fil: Jerez, Horacio Emanuel. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Area Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Simioni, Yamila Roxana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Area Química; Argentina
dc.description.fil
Fil: Ghosal, Kajal. Jadavpur University; India
dc.description.fil
Fil: Morilla, María José. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Area Química; Argentina
dc.description.fil
Fil: Romero, Eder Lilia. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Area Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.journal.title
Beilstein Journal of Nanotechnology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.beilstein-journals.org/bjnano/articles/15/46
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.3762/bjnano.15.46
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