Mostrar el registro sencillo del ítem
dc.contributor.author
Mentel, Maria Isabel
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.contributor.author
Loto, Flavia del Valle
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.contributor.author
Baigori, Mario Domingo
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.contributor.author
Pera, Licia Maria
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.date.available
2024-06-26T09:50:49Z
dc.date.issued
2024-03
dc.identifier.citation
Mentel, Maria Isabel; Loto, Flavia del Valle; Baigori, Mario Domingo; Pera, Licia Maria; A dye elution method for the quantification of insecticidal crystal proteins from Bacillus thuringiensis and its compatibility with the presence of agro-industrial raw materials and waste products; Oxford University Press; Letters in Applied Microbiology; 77; 3; 3-2024; 1-7
dc.identifier.issn
1472-765X
dc.identifier.uri
http://hdl.handle.net/11336/238497
dc.description.abstract
The insecticidal crystal proteins produced by Bacillus thuringiensis during sporulation are active ingredients against lepidopteran, dipteran, andcoleopteran insects. Several methods have been reported for their quantification, such as crystal counting, ELISA, and SDS-PAGE/densitometry.One of the major tasks in industrial processes is the analysis of raw material dependency and costs. Thus, the crystal protein quantificationmethod is expected to be compatible with the presence of complex and inexpensive culture medium components. This work presents a reval-idated elution-based method for the quantification of insecticidal crystal proteins produced by the native strain B. thuringiensis RT. To quantifyproteins, a calibration curve was generated by varying the amount of BSA loaded into SDS-PAGE gels. First, SDS-PAGE was performed for qual-ity control of the bioinsecticide. Then, the stained protein band was excised from 10% polyacrylamide gel and the protein-associated dye waseluted with an alcoholic solution of SDS (3% SDS in 50% isopropanol) during 45 min at 95◦C. This protocol was a sensitive procedure to quantifyproteins in the range of 2.0–10.0 μg. As proof of concept, proteins of samples obtained from a complex fermented broth were separated bySDS-PAGE. Then, Cry1 and Cry2 proteins were properly quantified.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Oxford University Press
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
BACILLUS THURINGIENSIS RT
dc.subject
CRISTAL PROTEIN
dc.subject
PAGE
dc.subject
DYE ELUTION
dc.subject
COMPLEX CULTURE MEDIUM
dc.subject.classification
Biología Celular, Microbiología
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.subject.classification
Ciencias Biológicas
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.title
A dye elution method for the quantification of insecticidal crystal proteins from Bacillus thuringiensis and its compatibility with the presence of agro-industrial raw materials and waste products
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2024-06-24T16:03:01Z
dc.journal.volume
77
dc.journal.number
3
dc.journal.pagination
1-7
dc.journal.pais
Reino Unido
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.journal.ciudad
Oxford
dc.description.fil
Fil: Mentel, Maria Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
dc.description.fil
Fil: Loto, Flavia del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
dc.description.fil
Fil: Baigori, Mario Domingo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
dc.description.fil
Fil: Pera, Licia Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
dc.journal.title
Letters in Applied Microbiology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/lambio/article-abstract/77/3/ovae024/7617697
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1093/lambio/ovae024
Archivos asociados