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dc.contributor.author
Crosio, Matias Ariel
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dc.contributor.author
Wilke, Natalia
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dc.date.available
2024-06-26T09:38:59Z
dc.date.issued
2024-03
dc.identifier.citation
Crosio, Matias Ariel; Wilke, Natalia; The neutral Red probe location in large unilamellar vesicles: Influence of membrane composition and phase states; Elsevier Science SA; Journal of Photochemistry and Photobiology A: Chemistry; 452; 3-2024; 1-9
dc.identifier.issn
1010-6030
dc.identifier.uri
http://hdl.handle.net/11336/238491
dc.description.abstract
Biological membranes are complex and dynamic self-assembled structures composed of lipids and proteins. They depict emergent properties that are heterogeneous in the membrane plane. Therefore, their study is currently one of the greatest challenges in biophysics.The versatility of spectroscopic techniques has encouraged the development of new fluorescent probes capable of sensing different membrane properties. The Neutral Red (NR) molecule is a dye that protonates at pH < 6.8 (pKa = 6.8), leading to the charged species (NRH+). NR is widely employed in molecular biology to assess cell viability and mitochondrial function, and to sense intracellular pH. However, the photophysical behavior of NR in complex media such as membranes were not systematically characterized even though of the wide application in molecular biology.In this work, the photophysical behavior of NNR (pH 8 neutral specie) and NRH+ (pH 4) was studied by employing large unilamellar vesicles (LUVs) with different compositions, membrane phase states.The results of this study show that the spectroscopic behavior of NNR is more sensitive to the membrane composition than that of NRH+. The wavelength of the maximum emission of NNR was at shorter wavelengths in liquid-disordered (Ld) membranes compared to gel or liquid-ordered (Lo) membranes. The Red-edge excitation shifts (REES) and Anisotropy magnitudes were lower in membranes in the gel or Lo, compared to membranes in Ld. The same tendency was observed for NRH+, although the spectral changes in this case were smaller, as well as the changes in the REES effect, and anisotropy magnitudes were lower related to the NR species. We suggest that NNR and NRH+ are localized in different regions of the membrane depending on the membrane phase estate.NR and NRH+ are located in more internal regions of the interface in membranes in Ld phase state, whereas in gel or Lo phase membranes, NR is located in a more external region, and NRH+ is adsorbed on the membrane surface. Consequently, the spectral signals of both, NNR and NRH+, correspond to the species distributed in different locations of the membrane in Ld compared to gel and Lo phase. Therefore, the information provided by the probe inserted in different membranes is not directly comparable.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science SA
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dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Membrane sensors
dc.subject
Vesicles
dc.subject
Phases states
dc.subject.classification
Biofísica
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
The neutral Red probe location in large unilamellar vesicles: Influence of membrane composition and phase states
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2024-06-25T10:39:37Z
dc.journal.volume
452
dc.journal.pagination
1-9
dc.journal.pais
Países Bajos
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dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Crosio, Matias Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
dc.description.fil
Fil: Wilke, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
dc.journal.title
Journal of Photochemistry and Photobiology A: Chemistry
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.jphotochem.2024.115615
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