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Artículo

Impaired spermatogenesis in infertile patients with orchitis and experimental autoimmune orchitis in rats

Amarilla, María SofíaIcon ; Glienke, Leilane; Munduruca Pires, Thaisy; Sobarzo, Cristian Marcelo; Oxilia, Hernán Gustavo; Fulco, María Florencia; Rodríguez Peña, Marcelo; Maio, María Belén; Ferrer Viñals, DenisseIcon ; Lustig, LiviaIcon ; Jacobo, Patricia VerónicaIcon ; Theas, Maria SusanaIcon
Fecha de publicación: 04/2024
Editorial: Multidisciplinary Digital Publishing Institute
Revista: Biology
ISSN: 2079-7737
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Otras Medicina Básica

Resumen

Experimental autoimmune orchitis (EAO) is a well-established rodent model of organ-specific autoimmunity associated with infertility in which the testis immunohistopathology has been extensively studied. In contrast, analysis of testis biopsies from infertile patients associated with inflammation has been more limited. In this work, testicular biopsies from patients with idiopathic non-obstructive azoospermia diagnosed with hypospermatogenesis (HypoSp) [mild: n = 9, and severe: n = 11], with obstructive azoospermia and complete Sp (spermatogenesis) (control group, C, n = 9), and from Sertoli cell-only syndrome (SCOS, n = 9) were analyzed for the presence of immune cells, spermatogonia and Sertoli cell (SCs) alterations, and reproductive hormones levels. These parameters were compared with those obtained in rats with EAO. The presence of increased CD45+ cells in the seminiferous tubules (STs) wall and lumen in severe HypoSp is associated with increased numbers of apoptotic meiotic germ cells and decreased populations of undifferentiated and differentiated spermatogonia. The SCs showed an immature profile with the highest expression of AMH in patients with SCOS and severe HypoSp. In SCOS patients, the amount of SCs/ST and Ki67+ SCs/ST increased and correlated with high serum FSH levels and CD45+ cells. In the severe phase of EAO, immune cell infiltration and apoptosis of meiotic germ cells increased and the number of undifferentiated and differentiated spermatogonia was lowest, as previously reported. Here, we found that orchitis leads to reduced sperm number, viability, and motility. SCs were mature (AMH-) but increased in number, with Ki67+ observed in severely damaged STs and associated with the highest levels of FSH and inflammatory cells. Our findings demonstrate that in a scenario where a chronic inflammatory process is underway, FSH levels, immune cell infiltration, and immature phenotypes of SCs are associated with severe changes in spermatogenesis, leading to azoospermia. Furthermore, AMH and Ki67 expression in SCs is a distinctive marker of severe alterations of STs in human orchitis.
Palabras clave: EXPERIMENTAL ORCHITIS , SERTOLI CELLS , MALE INFERTILITY , SPERMATOGENIA
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution 2.5 Unported (CC BY 2.5)
Identificadores
URI: http://hdl.handle.net/11336/238318
URL: https://www.mdpi.com/2079-7737/13/4/278
DOI: http://dx.doi.org/10.3390/biology13040278
Colecciones
Articulos(IBYME)
Articulos de INST.DE BIOLOGIA Y MEDICINA EXPERIMENTAL (I)
Articulos(INBIOMED)
Articulos de INSTITUTO DE INVESTIGACIONES BIOMEDICAS
Citación
Amarilla, María Sofía; Glienke, Leilane; Munduruca Pires, Thaisy; Sobarzo, Cristian Marcelo; Oxilia, Hernán Gustavo; et al.; Impaired spermatogenesis in infertile patients with orchitis and experimental autoimmune orchitis in rats; Multidisciplinary Digital Publishing Institute; Biology; 13; 4; 4-2024; 1-22
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