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dc.contributor.author
Adur, Javier Fernando
dc.contributor.author
D´Souza Li, Lilia
dc.contributor.author
Pedroni, Marcus Vinícius
dc.contributor.author
Steiner, Carlos E.
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Pelagati, Vitor B.
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de Thomaz, Andre A.
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Carvalho, Hernandes F.
dc.contributor.author
Cesar, Carlos L.
dc.date.available
2017-09-07T18:39:26Z
dc.date.issued
2013-07
dc.identifier.citation
Adur, Javier Fernando; D´Souza Li, Lilia; Pedroni, Marcus Vinícius; Steiner, Carlos E.; Pelagati, Vitor B.; et al.; The severity of Osteogenesis imperfecta and type I collagen pattern in human skin as determined by nonlinear microscopy: proof of principle of a diagnostic method; Public Library of Science; Plos One; 8; 7; 7-2013; 1-11
dc.identifier.issn
1932-6203
dc.identifier.uri
http://hdl.handle.net/11336/23785
dc.description.abstract
Background: The confirmatory diagnosis of Osteogenesis Imperfecta (OI) requires invasive, commonly bone biopsy, time consuming and destructive methods. This paper proposes an alternative method using a combination of two-photon excitation fluorescence (TPEF) and second-harmonic generation (SHG) microscopies from easily obtained human skin biopsies. We show that this method can distinguish subtypes of human OI. Methodology/Principal Findings: Different aspects of collagen microstructure of skin fresh biopsies and standard H&E-stained sections of normal and OI patients (mild and severe forms) were distinguished by TPEF and SHG images. Moreover, important differences between subtypes of OI were identified using different methods of quantification such as collagen density, ratio between collagen and elastic tissue, and gray-level co-occurrence matrix (GLCM) image-pattern analysis. Collagen density was lower in OI dermis, while the SHG/autofluorescence index of the dermis was significantly higher in OI as compared to that of the normal skin. We also showed that the energy value of GLCM texture analysis is useful to discriminate mild from severe OI and from normal skin. Conclusions/Significance: This work demonstrated that nonlinear microscopy techniques in combination with image-analysis approaches represent a powerful tool to investigate the collagen organization in skin dermis in patients with OI and has the potential to distinguish the different types of OI. The procedure outlined in this paper requires a skin biopsy, which is almost painless as compared to the bone biopsy commonly used in conventional methods. The data presented here complement existing clinical diagnostic techniques and can be used as a diagnostic procedure to confirm the disease, evaluate its severity and treatment efficacy.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Public Library of Science
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/
dc.subject
Osteogenesis Imperfecta
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Nonlinear Microscopy
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Texture Analysis
dc.subject.classification
Otras Ciencias Físicas
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Ciencias Físicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
The severity of Osteogenesis imperfecta and type I collagen pattern in human skin as determined by nonlinear microscopy: proof of principle of a diagnostic method
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-09-07T16:56:36Z
dc.journal.volume
8
dc.journal.number
7
dc.journal.pagination
1-11
dc.journal.pais
Estados Unidos
dc.journal.ciudad
San Francisco
dc.description.fil
Fil: Adur, Javier Fernando. Universidad Nacional de Entre Ríos. Facultad de Ingeniería; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidade Estadual de Campinas; Brasil
dc.description.fil
Fil: D´Souza Li, Lilia. Universidade Estadual de Campinas; Brasil
dc.description.fil
Fil: Pedroni, Marcus Vinícius. Universidade Estadual de Campinas; Brasil
dc.description.fil
Fil: Steiner, Carlos E.. Universidade Estadual de Campinas; Brasil
dc.description.fil
Fil: Pelagati, Vitor B.. Universidade Estadual de Campinas; Brasil
dc.description.fil
Fil: de Thomaz, Andre A.. Universidade Estadual de Campinas; Brasil
dc.description.fil
Fil: Carvalho, Hernandes F.. Universidade Estadual de Campinas; Brasil
dc.description.fil
Fil: Cesar, Carlos L.. Universidade Estadual de Campinas; Brasil
dc.journal.title
Plos One
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0069186
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1371/journal.pone.0069186
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