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dc.contributor.author
Pal, Pallabi
dc.contributor.author
Marañón, Rodrigo Oscar
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dc.contributor.author
Rivera Gonzales, Osvaldo J.
dc.contributor.author
Speed, Joshua S.
dc.contributor.author
Janorkar, Amol V.
dc.date.available
2024-06-11T11:40:57Z
dc.date.issued
2024-08
dc.identifier.citation
Pal, Pallabi; Marañón, Rodrigo Oscar; Rivera Gonzales, Osvaldo J.; Speed, Joshua S.; Janorkar, Amol V.; Sexual Dimorphism's impact on adipogenesis: A three-dimensional in vitro model treated with 17β-estradiol and testosterone; Elsevier Ireland; Molecular and Cellular Endocrinology; 589; 8-2024; 1-12
dc.identifier.issn
0303-7207
dc.identifier.uri
http://hdl.handle.net/11336/237757
dc.description.abstract
Using a three-dimensional (3-D) in vitro culture model, we report the dose dependent effect of 17β-estradiol and testosterone on the adipogenic differentiation and maturation of human adipose derived stem cells (hASCs) obtained from female and male patients. Considering sexual dimorphism, we expected male and female adipocytes to respond differently to the sex steroids. Both male and female hASC spheroids were exposed to 100 nM and 500 nM of 17β-estradiol and testosterone either at the beginning of the adipogenic maturation (Phase I) to discourage intracellular triglyceride accumulation or exposed after adipogenic maturation (Phase II) to reduce the intracellular triglyceride accumulation. The results show that 17β-estradiol leads to a dose dependent reduction in intracellular triglyceride accumulation in female hASC spheroids compared to the both untreated and testosterone-treated cells. Affirming our hypothesis, 17β-estradiol prevented intracellular triglyceride accumulation during Phase I, while it stimulated lipolysis during Phase II. PPAR-γ and adiponectin gene expression also reduced upon 17β-estradiol treatment in female cells. Interestingly, 17β-estradiol and testosterone had only a modest effect on the male hASC spheroids. Collectively, our findings suggest that 17β-estradiol can prevent fat accumulation in adipocytes during early and late stages of maturation in females.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Ireland
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dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Adipogenesis
dc.subject
Sex Differences
dc.subject
17betaestradiol
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Testosterone
dc.subject.classification
Fisiología
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dc.subject.classification
Medicina Básica
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dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
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dc.title
Sexual Dimorphism's impact on adipogenesis: A three-dimensional in vitro model treated with 17β-estradiol and testosterone
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2024-06-11T11:09:43Z
dc.journal.volume
589
dc.journal.pagination
1-12
dc.journal.pais
Irlanda
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dc.description.fil
Fil: Pal, Pallabi. University of Mississippi; Estados Unidos
dc.description.fil
Fil: Marañón, Rodrigo Oscar. Universidad Nacional de Tucumán. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina
dc.description.fil
Fil: Rivera Gonzales, Osvaldo J.. University of Mississippi; Estados Unidos
dc.description.fil
Fil: Speed, Joshua S.. University of Mississippi; Estados Unidos
dc.description.fil
Fil: Janorkar, Amol V.. University of Mississippi; Estados Unidos
dc.journal.title
Molecular and Cellular Endocrinology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.mce.2024.112249
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