Artículo
Designer transcription activator-like effectors enable discovery of cell death-inducer genes
Roeschlin, Roxana Andrea
; Azad, Sepideh; Grove, René P; Chuan, Ana; García, Lucila
; Niñoles, Regina; Uviedo, Facundo
; Villalobos, Liara; Massimino, Maria E.; Marano, María Rosa
; Boch, Jens; Gadea, José
Fecha de publicación:
05/2024
Editorial:
American Society of Plant Biologist
Revista:
Plant Physiology
ISSN:
0032-0889
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
TALEs (transcription activator-like effectors) in plant-pathogenic Xanthomonas bacteria activate expression of plant genes and support infection or cause a resistance response. PthA4AT is a TALE with a particularly short DNA-binding domain harbouring only 7.5-repeats which triggers cell death in Nicotiana benthamiana; however, the genetic basis for this remains unknown. To identify possible target genes of PthA4AT that mediate cell death in N. benthamiana, we exploited the modularity of TALEs to stepwise enhance their specificity and reduce potential target sites. Substitutions of individual repeats suggested that PthA4AT-dependent cell death is sequence-specific. Stepwise addition of repeats to the C-terminal or N-terminal end of the repeat region narrowed the sequence requirements in promoters of target genes. Transcriptome profiling and in silico target prediction allowed the isolation of two cell death-inducer genes, which encode a patatin-like protein and a bifunctional monodehydroascorbate reductase/carbonic anhydrase protein. These two proteins are not linked to known TALE-dependent resistance genes. Our results show that the aberrant expression of different endogenous plant genes can cause a cell death reaction, which supports the hypothesis that TALE-dependent executor resistance genes can originate from various plant processes. Our strategy further demonstrates the use of TALEs to scan genomes for genes triggering cell death and other relevant phenotypes.
Palabras clave:
TALE
,
cell-death
,
Resistanse genes
,
modularity
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Identificadores
Colecciones
Articulos(CCT - SANTA FE)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - SANTA FE
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - SANTA FE
Articulos(IBR)
Articulos de INST.DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Articulos de INST.DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Citación
Roeschlin, Roxana Andrea; Azad, Sepideh; Grove, René P; Chuan, Ana; García, Lucila; et al.; Designer transcription activator-like effectors enable discovery of cell death-inducer genes; American Society of Plant Biologist; Plant Physiology; 5-2024; 1-12
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